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Open AccessArticle

Characterization of PtAOS1 Promoter and Three Novel Interacting Proteins Responding to Drought in Poncirus trifoliata

Department of Horticulture, College of Horticulture, Hunan Agricultural University, Changsha 410128, China
National Centre for Citrus Improvement, Changsha 410128, China
Institute of Horticulture, Hunan Academy of Agricultural Science, Changsha 410125, China
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(13), 4705;
Received: 5 June 2020 / Revised: 27 June 2020 / Accepted: 29 June 2020 / Published: 1 July 2020
(This article belongs to the Section Molecular Plant Sciences)
Jasmonic acid (JA) plays a crucial role in various biological processes including development, signal transduction and stress response. Allene oxide synthase (AOS) catalyzing (13S)-hydroperoxyoctadecatrienoic acid (13-HPOT) to an unstable allene oxide is involved in the first step of JA biosynthesis. Here, we isolated the PtAOS1 gene and its promoter from trifoliate orange (Poncirus trifoliata). PtAOS1 contains a putative chloroplast targeting sequence in N-terminal and shows relative to pistachio (Pistacia vera) AOS. A number of stress-, light- and hormone-related cis-elements were found in the PtAOS1 promoter which may be responsible for the up-regulation of PtAOS1 under drought and JA treatments. Transient expression in tobacco (Nicotiana benthamiana) demonstrated that the P−532 (−532 to +1) fragment conferring drive activity was a core region in the PtAOS1 promoter. Using yeast one-hybrid, three novel proteins, PtDUF886, PtDUF1685 and PtRAP2.4, binding to P−532 were identified. The dual luciferase assay in tobacco illustrated that all three transcription factors could enhance PtAOS1 promoter activity. Genes PtDUF1685 and PtRAP2.4 shared an expression pattern which was induced significantly by drought stress. These findings should be available evidence for trifoliate orange responding to drought through JA modulation. View Full-Text
Keywords: allene oxide synthase; promoter; Poncirus trifoliata; interaction allene oxide synthase; promoter; Poncirus trifoliata; interaction
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Xiong, J.; Liu, L.; Ma, X.; Li, F.; Tang, C.; Li, Z.; Lü, B.; Zhou, T.; Lian, X.; Chang, Y.; Tang, M.; Xie, S.; Lu, X. Characterization of PtAOS1 Promoter and Three Novel Interacting Proteins Responding to Drought in Poncirus trifoliata. Int. J. Mol. Sci. 2020, 21, 4705.

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