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Silver Nanoparticles Surface-Modified with Carbosilane Dendrons as Carriers of Anticancer siRNA

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Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 141/143 Pomorska St., 90-236 Lodz, Poland
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Institute of Biophysics and Cell Engineering of NASB, 27 Akademicheskaya St., 220072 Minsk, Belarus
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Leibniz Institute of Polymer Research Dresden, 6 Hohe St., 01069 Dresden, Germany
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Department of Organic and Inorganic Chemistry and Research Chemistry Institute “Andrés M. del Río” (IQAR)., University of Alcalá, 28871 Alcalá de Henares, Spain
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Networking Research Center for Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain
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Institute “Ramón y Cajal” for Health Research (IRYCIS), 28034 Madrid, Spain
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Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(13), 4647; https://doi.org/10.3390/ijms21134647
Received: 29 April 2020 / Revised: 20 June 2020 / Accepted: 27 June 2020 / Published: 30 June 2020
(This article belongs to the Section Molecular Pharmacology)
Gene therapy is a promising approach in cancer treatment; however, current methods have a number of limitations mainly due to the difficulty in delivering therapeutic nucleic acids to their sites of action. The application of non-viral carriers based on nanomaterials aims at protecting genetic material from degradation and enabling its effective intracellular transport. We proposed the use of silver nanoparticles (AgNPs) surface-modified with carbosilane dendrons as carriers of anticancer siRNA (siBcl-xl). Using gel electrophoresis, zeta potential and hydrodynamic diameter measurements, as well as transmission electron microscopy, we characterized AgNP:siRNA complexes and demonstrated the stability of nucleic acid in complexes in the presence of RNase. Hemolytic properties of free silver nanoparticles and complexes, their effect on lymphocyte proliferation and cytotoxic activity on HeLa cells were also examined. Confocal microscopy proved the effective cellular uptake of complexes, indicating the possible use of this type of silver nanoparticles as carriers of genetic material in gene therapy. View Full-Text
Keywords: silver nanoparticles; carbosilane dendrons; siRNA; complexation; anticancer gene therapy silver nanoparticles; carbosilane dendrons; siRNA; complexation; anticancer gene therapy
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MDPI and ACS Style

Pędziwiatr-Werbicka, E.; Gorzkiewicz, M.; Horodecka, K.; Abashkin, V.; Klajnert-Maculewicz, B.; Peña-González, C.E.; Sánchez-Nieves, J.; Gómez, R.; de la Mata, F.J.; Bryszewska, M. Silver Nanoparticles Surface-Modified with Carbosilane Dendrons as Carriers of Anticancer siRNA. Int. J. Mol. Sci. 2020, 21, 4647. https://doi.org/10.3390/ijms21134647

AMA Style

Pędziwiatr-Werbicka E, Gorzkiewicz M, Horodecka K, Abashkin V, Klajnert-Maculewicz B, Peña-González CE, Sánchez-Nieves J, Gómez R, de la Mata FJ, Bryszewska M. Silver Nanoparticles Surface-Modified with Carbosilane Dendrons as Carriers of Anticancer siRNA. International Journal of Molecular Sciences. 2020; 21(13):4647. https://doi.org/10.3390/ijms21134647

Chicago/Turabian Style

Pędziwiatr-Werbicka, Elżbieta, Michał Gorzkiewicz, Katarzyna Horodecka, Viktar Abashkin, Barbara Klajnert-Maculewicz, Cornelia E. Peña-González, Javier Sánchez-Nieves, Rafael Gómez, F. J. de la Mata, and Maria Bryszewska. 2020. "Silver Nanoparticles Surface-Modified with Carbosilane Dendrons as Carriers of Anticancer siRNA" International Journal of Molecular Sciences 21, no. 13: 4647. https://doi.org/10.3390/ijms21134647

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