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Article

Functional Heterogeneity of Protein Kinase A Activation in Multipotent Stromal Cells

Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Lomonosov Moscow State University, 119991 Moscow, Russia
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Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(12), 4442; https://doi.org/10.3390/ijms21124442
Received: 30 May 2020 / Revised: 17 June 2020 / Accepted: 17 June 2020 / Published: 22 June 2020
(This article belongs to the Special Issue Kinase Signal Transduction 2.0)
Multipotent stromal cells (MSC) demonstrate remarkable functional heterogeneity; however, its molecular mechanisms remain largely obscure. In this study, we explored MSC response to hormones, which activate Gs-protein / cyclic AMP (cAMP) / protein kinase A (PKA) dependent signaling, at the single cell level using genetically encoded biosensor PKA-Spark. For the first time, we demonstrated that about half of cultured MSCs are not able to activate the cAMP/PKA pathway, possibly due to the limited availability of adenylyl cyclases. Using this approach, we showed that MSC subpopulations responding to various hormones largely overlapped, and the share of responding cells did not exceed 40%. Using clonal analysis, we showed that signaling heterogeneity of MSC could be formed de novo within 2 weeks. View Full-Text
Keywords: multipotent stromal cells; mesenchymal stem/stromal cells; protein kinase A; functional heterogeneity; single cell analysis; intracellular signaling; PKA-Spark biosensor multipotent stromal cells; mesenchymal stem/stromal cells; protein kinase A; functional heterogeneity; single cell analysis; intracellular signaling; PKA-Spark biosensor
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MDPI and ACS Style

Tyurin-Kuzmin, P.A.; Karagyaur, M.N.; Kulebyakin, K.Y.; Dyikanov, D.T.; Chechekhin, V.I.; Ivanova, A.M.; Skryabina, M.N.; Arbatskiy, M.S.; Sysoeva, V.Y.; Kalinina, N.I.; Tkachuk, V.A. Functional Heterogeneity of Protein Kinase A Activation in Multipotent Stromal Cells. Int. J. Mol. Sci. 2020, 21, 4442. https://doi.org/10.3390/ijms21124442

AMA Style

Tyurin-Kuzmin PA, Karagyaur MN, Kulebyakin KY, Dyikanov DT, Chechekhin VI, Ivanova AM, Skryabina MN, Arbatskiy MS, Sysoeva VY, Kalinina NI, Tkachuk VA. Functional Heterogeneity of Protein Kinase A Activation in Multipotent Stromal Cells. International Journal of Molecular Sciences. 2020; 21(12):4442. https://doi.org/10.3390/ijms21124442

Chicago/Turabian Style

Tyurin-Kuzmin, Pyotr A., Maxim N. Karagyaur, Konstantin Yu. Kulebyakin, Daniyar T. Dyikanov, Vadim I. Chechekhin, Anastasiya M. Ivanova, Mariya N. Skryabina, Mikhail S. Arbatskiy, Veronika Yu. Sysoeva, Natalia I. Kalinina, and Vsevolod A. Tkachuk. 2020. "Functional Heterogeneity of Protein Kinase A Activation in Multipotent Stromal Cells" International Journal of Molecular Sciences 21, no. 12: 4442. https://doi.org/10.3390/ijms21124442

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