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Open AccessArticle

Molecular and Functional Phenotypes of Human Bone Marrow-Derived Mesenchymal Stromal Cells Depend on Harvesting Techniques

1
Clinic for Orthopedics and Trauma Surgery, University Hospital Bonn, 53127 Bonn, Germany
2
Clinic for Cardiothoracic Surgery, University Hospital Cologne, 50937 Cologne, Germany
3
Department of Orthopaedics and Trauma Surgery, University Medical Center Mannheim of University Heidelberg, 68167 Mannheim, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(12), 4382; https://doi.org/10.3390/ijms21124382
Received: 29 April 2020 / Revised: 30 May 2020 / Accepted: 17 June 2020 / Published: 19 June 2020
(This article belongs to the Special Issue Biological Basis of Musculoskeletal Regeneration 2019)
Mesenchymal stromal cells (MSC) harvested in different tissues from the same donor exhibit different phenotypes. Each phenotype is not only characterized by a certain pattern of cell surface markers, but also different cellular functionalities. Only recently were different harvesting and processing techniques found to contribute to this phenomenon as well. This study was therefore set up to investigate proteomic and functional properties of human bone marrow-derived MSCs (hBM-MSC). These were taken from the same tissue and donor site but harvested either as aspirate or bone chip cultures. Both MSC populations were profiled for MSC markers defined by the International Society for Cellular Therapy (ISCT), MSC markers currently under discussion and markers of particular interest. While classic ISCT MSC markers did not show any significant difference between aspirate and outgrowth hBM-MSCs, our additional characterization panel revealed distinct patterns of differentially expressed markers. Furthermore, hBM-MSCs from aspirate cultures demonstrated a significantly higher osteogenic differentiation potential than outgrowth MSCs, which could be confirmed using a transcriptional approach. Our comparison of MSC phenotypes obtained by different harvesting techniques suggests the need of future standardized harvesting, processing and phenotyping procedures in order to gain better comparability in the MSC field. View Full-Text
Keywords: mesenchymal stromal cells; phenotype; characterization; differentiation; harvesting technique; osteoimmunology mesenchymal stromal cells; phenotype; characterization; differentiation; harvesting technique; osteoimmunology
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MDPI and ACS Style

Walter, S.G.; Randau, T.M.; Hilgers, C.; Haddouti, E.-M.; Masson, W.; Gravius, S.; Burger, C.; Wirtz, D.C.; Schildberg, F.A. Molecular and Functional Phenotypes of Human Bone Marrow-Derived Mesenchymal Stromal Cells Depend on Harvesting Techniques. Int. J. Mol. Sci. 2020, 21, 4382.

AMA Style

Walter SG, Randau TM, Hilgers C, Haddouti E-M, Masson W, Gravius S, Burger C, Wirtz DC, Schildberg FA. Molecular and Functional Phenotypes of Human Bone Marrow-Derived Mesenchymal Stromal Cells Depend on Harvesting Techniques. International Journal of Molecular Sciences. 2020; 21(12):4382.

Chicago/Turabian Style

Walter, Sebastian G.; Randau, Thomas M.; Hilgers, Cäcilia; Haddouti, El-Mustapha; Masson, Werner; Gravius, Sascha; Burger, Christof; Wirtz, Dieter C.; Schildberg, Frank A. 2020. "Molecular and Functional Phenotypes of Human Bone Marrow-Derived Mesenchymal Stromal Cells Depend on Harvesting Techniques" Int. J. Mol. Sci. 21, no. 12: 4382.

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