Next Article in Journal
Insights about MYC and Apoptosis in B-Lymphomagenesis: An Update from Murine Models
Next Article in Special Issue
Functional Markers for Precision Plant Breeding
Previous Article in Journal
Exosomes as Messengers between Mother and Fetus in Pregnancy
Article

Mining for Candidate Genes Controlling Secondary Growth of the Carrot Storage Root

1
Department of Plant Biology and Biotechnology, University of Agriculture in Krakow, 31-120 Krakow, Poland
2
Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA
3
USDA ARS Vegetable Crops Research Unit, University of Wisconsin-Madison, Madison, WI 53706, USA
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(12), 4263; https://doi.org/10.3390/ijms21124263
Received: 27 May 2020 / Revised: 10 June 2020 / Accepted: 12 June 2020 / Published: 15 June 2020
(This article belongs to the Special Issue Functional Genomics for Plant Breeding)
Background: Diverse groups of carrot cultivars have been developed to meet consumer demands and industry needs. Varietal groups of the cultivated carrot are defined based on the shape of roots. However, little is known about the genetic basis of root shape determination. Methods: Here, we used 307 carrot plants from 103 open-pollinated cultivars for a genome wide association study to identify genomic regions associated with the storage root morphology. Results: A 180 kb-long region on carrot chromosome 1 explained 10% of the total observed phenotypic variance in the shoulder diameter. Within that region, DcDCAF1 and DcBTAF1 genes were proposed as candidates controlling secondary growth of the carrot storage root. Their expression profiles differed between the cultivated and the wild carrots, likely indicating that their elevated expression was required for the development of edible roots. They also showed higher expression at the secondary root growth stage in cultivars producing thick roots, as compared to those developing thin roots. Conclusions: We provided evidence for a likely involvement of DcDCAF1 and/or DcBTAF1 in the development of the carrot storage root and developed a genotyping assay facilitating the identification of variants in the region on carrot chromosome 1 associated with secondary growth of the carrot root. View Full-Text
Keywords: association mapping; Daucus carota; DCAF1; BTAF1; root shape; storage root association mapping; Daucus carota; DCAF1; BTAF1; root shape; storage root
Show Figures

Figure 1

MDPI and ACS Style

Macko-Podgórni, A.; Stelmach, K.; Kwolek, K.; Machaj, G.; Ellison, S.; Senalik, D.A.; Simon, P.W.; Grzebelus, D. Mining for Candidate Genes Controlling Secondary Growth of the Carrot Storage Root. Int. J. Mol. Sci. 2020, 21, 4263. https://doi.org/10.3390/ijms21124263

AMA Style

Macko-Podgórni A, Stelmach K, Kwolek K, Machaj G, Ellison S, Senalik DA, Simon PW, Grzebelus D. Mining for Candidate Genes Controlling Secondary Growth of the Carrot Storage Root. International Journal of Molecular Sciences. 2020; 21(12):4263. https://doi.org/10.3390/ijms21124263

Chicago/Turabian Style

Macko-Podgórni, Alicja, Katarzyna Stelmach, Kornelia Kwolek, Gabriela Machaj, Shelby Ellison, Douglas A. Senalik, Philipp W. Simon, and Dariusz Grzebelus. 2020. "Mining for Candidate Genes Controlling Secondary Growth of the Carrot Storage Root" International Journal of Molecular Sciences 21, no. 12: 4263. https://doi.org/10.3390/ijms21124263

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop