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Article

New Determinants of Aminoglycoside Resistance and Their Association with the Class 1 Integron Gene Cassettes in Trueperella pyogenes

Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8 St., 02-786 Warsaw, Poland
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Int. J. Mol. Sci. 2020, 21(12), 4230; https://doi.org/10.3390/ijms21124230
Received: 26 May 2020 / Revised: 7 June 2020 / Accepted: 11 June 2020 / Published: 13 June 2020
(This article belongs to the Special Issue Drug Resistance Mechanisms in Bacteria)
Trueperella pyogenes is an important opportunistic animal pathogen. Different antimicrobials, including aminoglycosides, are used to treat T. pyogenes infections. The aim of the present study was to evaluate aminoglycoside susceptibility and to detect aminoglycoside resistance determinants in 86 T. pyogenes isolates of different origin. Minimum inhibitory concentration of gentamicin, streptomycin, and kanamycin was determined using a standard broth microdilution method. Genetic elements associated with aminoglycoside resistance were investigated by PCR and DNA sequencing. All studied isolates were susceptible to gentamicin, but 32.6% and 11.6% of them were classified as resistant to streptomycin and kanamycin, respectively. A total of 30 (34.9%) isolates contained class 1 integrons. Class 1 integron gene cassettes carrying aminoglycoside resistance genes, aadA11 and aadA9, were found in seven and two isolates, respectively. Additionally, the aadA9 gene found in six isolates was not associated with mobile genetic elements. Moreover, other, not carried by gene cassettes, aminoglycoside resistance genes, strA-strB and aph(3’)-IIIa, were also detected. Most importantly, this is the first description of all reported genes in T. pyogenes. Nevertheless, the relevance of the resistance phenotype to genotype was not perfectly matched in 14 isolates. Therefore, further investigations are needed to fully explain aminoglycoside resistance mechanisms in T. pyogenes. View Full-Text
Keywords: aminoglycosides; antimicrobial resistance; gene cassette; integron; pathogen; Trueperella pyogenes aminoglycosides; antimicrobial resistance; gene cassette; integron; pathogen; Trueperella pyogenes
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MDPI and ACS Style

Kwiecień, E.; Stefańska, I.; Chrobak-Chmiel, D.; Sałamaszyńska-Guz, A.; Rzewuska, M. New Determinants of Aminoglycoside Resistance and Their Association with the Class 1 Integron Gene Cassettes in Trueperella pyogenes. Int. J. Mol. Sci. 2020, 21, 4230. https://doi.org/10.3390/ijms21124230

AMA Style

Kwiecień E, Stefańska I, Chrobak-Chmiel D, Sałamaszyńska-Guz A, Rzewuska M. New Determinants of Aminoglycoside Resistance and Their Association with the Class 1 Integron Gene Cassettes in Trueperella pyogenes. International Journal of Molecular Sciences. 2020; 21(12):4230. https://doi.org/10.3390/ijms21124230

Chicago/Turabian Style

Kwiecień, Ewelina, Ilona Stefańska, Dorota Chrobak-Chmiel, Agnieszka Sałamaszyńska-Guz, and Magdalena Rzewuska. 2020. "New Determinants of Aminoglycoside Resistance and Their Association with the Class 1 Integron Gene Cassettes in Trueperella pyogenes" International Journal of Molecular Sciences 21, no. 12: 4230. https://doi.org/10.3390/ijms21124230

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