Cell Adhesion Molecule 1 Contributes to Cell Survival in Crowded Epithelial Monolayers
Abstract
:1. Introduction
2. Results
2.1. CADM1 Expression Increases as Epithelial Cells Crowd
2.2. CADM1 Knockdown Induces Apoptosis in Crowded Epithelial Cells
2.3. 9D2 Decreases CADM1 Levels and Induces Apoptosis in Crowded Epithelial Cells
3. Discussion
4. Materials and Methods
4.1. Cell Lines
4.2. Antibodies
4.3. Cell Culture
4.4. Electroporation
4.5. Western Blot Analysis
4.6. Immunofluorescence and Confocal Microscopy
4.7. 9D2 Treatment and Apoptosis Detection
4.8. RT-PCR
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
2D | 2-dimentional |
ANOVA | analysis of variance |
DAPI | 4′,6-diamidino-2-phenylindole |
HEPES | 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid |
TUNEL | terminal deoxynucleotidyl transferase-mediated dNTP-biotin nick end labeling of DNA fragments |
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Hagiyama, M.; Kimura, R.; Yoneshige, A.; Inoue, T.; Otani, T.; Ito, A. Cell Adhesion Molecule 1 Contributes to Cell Survival in Crowded Epithelial Monolayers. Int. J. Mol. Sci. 2020, 21, 4123. https://doi.org/10.3390/ijms21114123
Hagiyama M, Kimura R, Yoneshige A, Inoue T, Otani T, Ito A. Cell Adhesion Molecule 1 Contributes to Cell Survival in Crowded Epithelial Monolayers. International Journal of Molecular Sciences. 2020; 21(11):4123. https://doi.org/10.3390/ijms21114123
Chicago/Turabian StyleHagiyama, Man, Ryuichiro Kimura, Azusa Yoneshige, Takao Inoue, Tomoyuki Otani, and Akihiko Ito. 2020. "Cell Adhesion Molecule 1 Contributes to Cell Survival in Crowded Epithelial Monolayers" International Journal of Molecular Sciences 21, no. 11: 4123. https://doi.org/10.3390/ijms21114123