Next Article in Journal
Wound Trauma Exacerbates Acute, but not Delayed, Effects of Radiation in Rats: Mitigation by Lisinopril
Next Article in Special Issue
Ablation of the miRNA Cluster 24 Has Profound Effects on Extracellular Matrix Protein Abundance in Cartilage
Previous Article in Journal
Albendazole-Induced SIRT3 Upregulation Protects Human Leukemia K562 Cells from the Cytotoxicity of MCL1 Suppression
Previous Article in Special Issue
Implications of Skeletal Muscle Extracellular Matrix Remodeling in Metabolic Disorders: Diabetes Perspective

Extracellular Matrix Analysis of Human Renal Arteries in Both Quiescent and Active Vascular State

Department of Nephrology and Hypertension, Division of Internal Medicine and Dermatology, University Medical Center Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands
Center for Proteomics, Erasmus Medical Center, 3015 CN Rotterdam, The Netherlands
Department of Pathology, Erasmus University Medical Center, 3015 CN Rotterdam, The Netherlands
Department of Cell and Chemical Biology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
Experimental Cardiology, Department of Cardiology, Thorax center, Erasmus MC, University Medical Center Rotterdam, PO Box 2040, 3000 CA Rotterdam, The Netherlands
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(11), 3905;
Received: 4 May 2020 / Revised: 27 May 2020 / Accepted: 29 May 2020 / Published: 30 May 2020
(This article belongs to the Special Issue Extracellular Matrix in Development and Disease 2.0)
In vascular tissue engineering strategies, the addition of vascular-specific extracellular matrix (ECM) components may better mimic the in vivo microenvironment and potentially enhance cell–matrix interactions and subsequent tissue growth. For this purpose, the exact composition of the human vascular ECM first needs to be fully characterized. Most research has focused on characterizing ECM components in mature vascular tissue; however, the developing fetal ECM matches the active environment required in vascular tissue engineering more closely. Consequently, we characterized the ECM protein composition of active (fetal) and quiescent (mature) renal arteries using a proteome analysis of decellularized tissue. The obtained human fetal renal artery ECM proteome dataset contains higher levels of 15 ECM proteins versus the mature renal artery ECM proteome, whereas 16 ECM proteins showed higher levels in the mature tissue compared to fetal. Elastic ECM proteins EMILIN1 and FBN1 are significantly enriched in fetal renal arteries and are mainly produced by cells of mesenchymal origin. We functionally tested the role of EMILIN1 and FBN1 by anchoring the ECM secreted by vascular smooth muscle cells (SMCs) to glass coverslips. This ECM layer was depleted from either EMILIN1 or FBN1 by using siRNA targeting of the SMCs. Cultured endothelial cells (ECs) on this modified ECM layer showed alterations on the transcriptome level of multiple pathways, especially the Rho GTPase controlled pathways. However, no significant alterations in adhesion, migration or proliferation were observed when ECs were cultured on EMILIN1- or FNB1-deficient ECM. To conclude, the proteome analysis identified unique ECM proteins involved in the embryonic development of renal arteries. Alterations in transcriptome levels of ECs cultured on EMILIN1- or FBN1-deficient ECM showed that these candidate proteins could affect the endothelial (regenerative) response. View Full-Text
Keywords: extracellular matrix; proteomics; EMILIN1; FBN1; vasculature extracellular matrix; proteomics; EMILIN1; FBN1; vasculature
Show Figures

Graphical abstract

MDPI and ACS Style

van Dijk, C.G.M.; Louzao-Martinez, L.; van Mulligen, E.; Boermans, B.; Demmers, J.A.A.; van den Bosch, T.P.P.; Goumans, M.-J.; Duncker, D.J.; Verhaar, M.C.; Cheng, C. Extracellular Matrix Analysis of Human Renal Arteries in Both Quiescent and Active Vascular State. Int. J. Mol. Sci. 2020, 21, 3905.

AMA Style

van Dijk CGM, Louzao-Martinez L, van Mulligen E, Boermans B, Demmers JAA, van den Bosch TPP, Goumans M-J, Duncker DJ, Verhaar MC, Cheng C. Extracellular Matrix Analysis of Human Renal Arteries in Both Quiescent and Active Vascular State. International Journal of Molecular Sciences. 2020; 21(11):3905.

Chicago/Turabian Style

van Dijk, Christian G.M., Laura Louzao-Martinez, Elise van Mulligen, Bart Boermans, Jeroen A.A. Demmers, Thierry P.P. van den Bosch, Marie-José Goumans, Dirk J. Duncker, Marianne C. Verhaar, and Caroline Cheng. 2020. "Extracellular Matrix Analysis of Human Renal Arteries in Both Quiescent and Active Vascular State" International Journal of Molecular Sciences 21, no. 11: 3905.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop