Next Article in Journal
Oncogenic Potential of Bisphenol A and Common Environmental Contaminants in Human Mammary Epithelial Cells
Next Article in Special Issue
Physicochemical Evidence that Francisella FupA and FupB Proteins Are Porins
Previous Article in Journal
Adherent-Invasive E. coli: Update on the Lifestyle of a Troublemaker in Crohn’s Disease
Previous Article in Special Issue
FGCaMP7, an Improved Version of Fungi-Based Ratiometric Calcium Indicator for In Vivo Visualization of Neuronal Activity
Article

Mitochondrial pH Nanosensors for Metabolic Profiling of Breast Cancer Cell Lines

1
Departamento de Fisicoquimica, Facultad de Farmacia, Unidad de Excelencia en Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, Campus Cartuja, 18071 Granada, Spain
2
GENYO, Pfizer-Universidad de Granada-Junta de Andalucia Centre for Genomics and Oncological Research, Avda Ilustracion 114, PTS, 18016 Granada, Spain
3
Departamento de Quimica Organica, Facultad de Ciencias, Unidad de Excelencia en Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, Campus Fuentenueva, 18071 Granada, Spain
4
Departamento de Quimica Farmaceutica y Organica, Facultad de Farmacia, Unidad de Excelencia en Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, Campus Cartuja, 18071 Granada, Spain
5
Departamento de Bioquimica y Biologia Celular I, Facultad de Ciencias, Universidad de Granada, Campus Fuentenueva, 18071 Granada, Spain
*
Author to whom correspondence should be addressed.
Current affiliation: Laboratorio de Estudios Cristalográficos, Instituto Andaluz de Ciencias de la Tierra, CSIC. Avda, De las Palmeras 4, 18100 Armilla, Spain.
Current affiliation: Department of Chemistry, Durham University, South Road, Durham DH1 3LE, UK.
Int. J. Mol. Sci. 2020, 21(10), 3731; https://doi.org/10.3390/ijms21103731
Received: 4 May 2020 / Revised: 21 May 2020 / Accepted: 22 May 2020 / Published: 25 May 2020
The main role of mitochondria, as pivotal organelles for cellular metabolism, is the production of energy (ATP) through an oxidative phosphorylation system. During this process, the electron transport chain creates a proton gradient that drives the synthesis of ATP. One of the main features of tumoral cells is their altered metabolism, providing alternative routes to enhance proliferation and survival. Hence, it is of utmost importance to understand the relationship between mitochondrial pH, tumoral metabolism, and cancer. In this manuscript, we develop a highly specific nanosensor to accurately measure the intramitochondrial pH using fluorescence lifetime imaging microscopy (FLIM). Importantly, we have applied this nanosensor to establish differences that may be hallmarks of different metabolic pathways in breast cancer cell models, leading to the characterization of different metabophenotypes. View Full-Text
Keywords: FLIM microscopy; nanosensing; intracellular sensors; tumoral metabolism; cancer metabolism FLIM microscopy; nanosensing; intracellular sensors; tumoral metabolism; cancer metabolism
Show Figures

Graphical abstract

MDPI and ACS Style

Ripoll, C.; Roldan, M.; Contreras-Montoya, R.; Diaz-Mochon, J.J.; Martin, M.; Ruedas-Rama, M.J.; Orte, A. Mitochondrial pH Nanosensors for Metabolic Profiling of Breast Cancer Cell Lines. Int. J. Mol. Sci. 2020, 21, 3731. https://doi.org/10.3390/ijms21103731

AMA Style

Ripoll C, Roldan M, Contreras-Montoya R, Diaz-Mochon JJ, Martin M, Ruedas-Rama MJ, Orte A. Mitochondrial pH Nanosensors for Metabolic Profiling of Breast Cancer Cell Lines. International Journal of Molecular Sciences. 2020; 21(10):3731. https://doi.org/10.3390/ijms21103731

Chicago/Turabian Style

Ripoll, Consuelo, Mar Roldan, Rafael Contreras-Montoya, Juan J. Diaz-Mochon, Miguel Martin, Maria J. Ruedas-Rama, and Angel Orte. 2020. "Mitochondrial pH Nanosensors for Metabolic Profiling of Breast Cancer Cell Lines" International Journal of Molecular Sciences 21, no. 10: 3731. https://doi.org/10.3390/ijms21103731

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop