Overexpression of Melon Tonoplast Sugar Transporter CmTST1 Improved Root Growth under High Sugar Content
Abstract
:1. Introduction
2. Results
2.1. CmTST1 Is Highly Expressed in the Roots
2.2. Expression of CmTST1 Is Induced by Sugar in the Roots
2.3. Ectopic Overexpression of CmTST1 in Arabidopsis Improves Root Growth under High Sugar Stress
2.4. Ectopic Overexpression of CmTST1 in Arabidopsis Affects the Expression of SUC and STP Genes
3. Discussion
3.1. Tonoplast Sugar Transporter in the Roots
3.2. Expression of CmTST1 Is Induced by Sugar
3.3. CmTST1 Plays an Important Role in the Root
3.4. Overexpression of CmTST1 in Plant Promotes the Expression of Plasma Membrane-Located Sugar Transporters
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. RNA Isolation, RT-PCR, and qRT-PCR
4.3. Transient Transformation and GUS Staining in Melon Roots
4.4. Sugar Induction Experiment
4.5. Generation of CmTST1 Overexpression Mutants
4.6. Bioinformatics Analysis and Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Lu, B.; Wen, S.; Zhu, P.; Cao, H.; Zhou, Y.; Bie, Z.; Cheng, J. Overexpression of Melon Tonoplast Sugar Transporter CmTST1 Improved Root Growth under High Sugar Content. Int. J. Mol. Sci. 2020, 21, 3524. https://doi.org/10.3390/ijms21103524
Lu B, Wen S, Zhu P, Cao H, Zhou Y, Bie Z, Cheng J. Overexpression of Melon Tonoplast Sugar Transporter CmTST1 Improved Root Growth under High Sugar Content. International Journal of Molecular Sciences. 2020; 21(10):3524. https://doi.org/10.3390/ijms21103524
Chicago/Turabian StyleLu, Baiyi, Suying Wen, Peilu Zhu, Haishun Cao, Yixuan Zhou, Zhilong Bie, and Jintao Cheng. 2020. "Overexpression of Melon Tonoplast Sugar Transporter CmTST1 Improved Root Growth under High Sugar Content" International Journal of Molecular Sciences 21, no. 10: 3524. https://doi.org/10.3390/ijms21103524