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Open AccessArticle

The Effects of High Molecular Weight Hyaluronic Acid Eye Drop Application in Environmental Dry Eye Stress Model Mice

1
Department of Ophthalmology, Keio University School of Medicine, Tokyo 160-8582, Japan
2
i.com medical GmbH, 81241 Munich, Germany
3
Karolinska Institutet, Dept of Clin NeuroSci, St Eriks Eye Hospital, 11282 Stockholm, Sweden
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(10), 3516; https://doi.org/10.3390/ijms21103516
Received: 3 April 2020 / Revised: 12 May 2020 / Accepted: 13 May 2020 / Published: 15 May 2020
(This article belongs to the Special Issue Dry Eye and Ocular Surface Disorders 2.0)
Hyaluronic acid (HA) ophthalmic solution is widely used in dry eye treatment worldwide. However, there are no reports comparing the dry eye treatment effects of high molecular weight HA with low molecular weight HA. Sixty eight-week-old C57BL/6 mice were assigned to the following 6 groups and exposed to environmental dry eye stress (EDES) that mimics office work environment: (1) 0.1% low molecular weight HA (LMWHA) eye drops, (2) 0.3% LMWHA eye drops, (3) 3% diquafosol sodium (DQ) eye drops, (4) 0.15% high molecular weight HA (HMWHA) eye drops, (5) no treatment with exposure to EDES (EDES+/Treatment−), and (6) no treatment without exposure to EDES (EDES−/Treatment−). After EDES, the HMWHA group had significantly longer break-up time (BUT) than the 0.1%, 0.3% LMWHA groups and the DQ group. After EDES, the HMWHA group had significantly lower lissamine green staining scores than the LMWHA and DQ groups. Subepithelial presumed dendritic cell density in the HMWHA group was significantly lower than the EDES+/Treatment− group. After EDES exposure, Conjunctival Muc5AC mRNA expression in the HMWHA group was significantly higher than the 0.1 and 0.3% LMWHA groups. Ophthalmic HMWHA solution may have a better dry eye treatment effect than LMWHA or DQ solution, owing to its anti-inflammatory effect. View Full-Text
Keywords: dry eye; high molecular weight hyaluronic acid; treatment; corneal subbasal dendritic cell density; Muc5AC; ocular surface staining dry eye; high molecular weight hyaluronic acid; treatment; corneal subbasal dendritic cell density; Muc5AC; ocular surface staining
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MDPI and ACS Style

Kojima, T.; Nagata, T.; Kudo, H.; Müller-Lierheim, W.G.K.; van Setten, G.-B.; Dogru, M.; Tsubota, K. The Effects of High Molecular Weight Hyaluronic Acid Eye Drop Application in Environmental Dry Eye Stress Model Mice. Int. J. Mol. Sci. 2020, 21, 3516. https://doi.org/10.3390/ijms21103516

AMA Style

Kojima T, Nagata T, Kudo H, Müller-Lierheim WGK, van Setten G-B, Dogru M, Tsubota K. The Effects of High Molecular Weight Hyaluronic Acid Eye Drop Application in Environmental Dry Eye Stress Model Mice. International Journal of Molecular Sciences. 2020; 21(10):3516. https://doi.org/10.3390/ijms21103516

Chicago/Turabian Style

Kojima, Takashi; Nagata, Taeko; Kudo, Haruka; Müller-Lierheim, Wolfgang G.K.; van Setten, Gysbert-Botho; Dogru, Murat; Tsubota, Kazuo. 2020. "The Effects of High Molecular Weight Hyaluronic Acid Eye Drop Application in Environmental Dry Eye Stress Model Mice" Int. J. Mol. Sci. 21, no. 10: 3516. https://doi.org/10.3390/ijms21103516

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