Immunohistochemical Analysis Revealed a Correlation between Musashi-2 and Cyclin-D1 Expression in Patients with Oral Squamous Cells Carcinoma
Abstract
1. Introduction
2. Results
2.1. Analysis of MSI2 Mutations, Gene Methylation and mRNA Expression in TCGA Database
2.2. Immunohistochemical Analysis of MSI2 Expression on TMA
3. Discussion
4. Methods and Materials
4.1. Analysis of MSI2 Expression and Methylation in The Cancer Genome Atlas (TCGA)
4.2. Immunohistochemistry of MSI2 Expression in OSCC Tissue Microarray
4.3. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Clinic-Pathological Information | Groups | Number of Patients |
---|---|---|
Age | ≤ 65 years old | 144/241 |
> 65 years old | 97/241 | |
Gender | Male | 158/241 |
Female | 83/241 | |
Grade | 1 | 43/241 |
2 3 | 147/241 51/241 | |
Stage | 1–2 | 76/241 |
3–4 | 165/241 | |
Subsite | Tongue | 103/241 |
Gingivo-buccal | 30/241 | |
Floor of the mouth | 46/241 | |
Others | 62/241 |
Variable | Age | Grade | Stage | Gender | Perineural Invasion | MSI2 Methylation | MSI2 mRNA Expression | Ki-67 mRNA Expression | Cyclin-D mRNA Expression |
---|---|---|---|---|---|---|---|---|---|
Age | ρ = 1 | 0.088 | −0.084 | 0.243 | 0.060 | 0.140 | −0.121 | −0.069 | 0.033 |
p-value = 1 | 0.175 | 0.197 | 0.001 ** | 0.414 | 0.03 * | 0.06 | 0.287 | 0.607 | |
Grade | ρ = 1 | −0.003 | −0.066 | 0.100 | −0.094 | 0.169 | −0.105 | 0.053 | |
p-value = 1 | 0.959 | 0.307 | 0.176 | 0.146 | 0.009 ** | 0.105 | 0.418 | ||
Stage | ρ = 1 | −0.026 | 0.199 | −0.031 | −0.035 | 0.038 | −0.062 | ||
p-value = 1 | 0.686 | 0.006 ** | 0.632 | 0.587 | 0.561 | 0.340 | |||
Gender | ρ = 1 | −0.090 | −0.045 | −0.220 | 0.042 | 0.043 | |||
p-value = 1 | 0.907 | 0.485 | 0.001 ** | 0.520 | 0.505 | ||||
Perineural Invasion | ρ = 1 | −0.178 | 0.033 | −0.132 | −0.038 | ||||
p-value = 1 | 0.014 * | 0.650 | 0.071 | 0.606 | |||||
MSI2 Methylation | ρ = 1 | −0.044 | 0.094 | 0.027 | |||||
p-value = 1 | 0.498 | 0.144 | 0.673 | ||||||
MSI2 mRNA expression | ρ = 1 | −0.093 | 0.003 | ||||||
p-value = 1 | 0.150 | 0.963 | |||||||
Ki-67 mRNA expression | ρ = 1 | 0.253 | |||||||
p-value = 1 | 0.000 ** | ||||||||
Cyclin-D mRNA expression | ρ = 1 | ||||||||
p-value = 1 |
Clinic-Pathological Information | Groups | Number of Patients |
---|---|---|
Age | ≤ 65 years old | 45/108 |
> 65 years old | 63/108 | |
Gender | Male | 79/108 |
Female | 29/108 | |
Grade | 1 2 | 22/108 50/108 |
3 | 36/108 | |
Stage | 1–2 | 35/108 |
3–4 | 73/108 | |
Subsite | Tongue | 67/108 |
Gingivo-buccal | 23/108 | |
Floor of the mouth | 13/108 | |
Others | 5/108 |
Variable | Age | Grade | Stage | Gender | MSI2 Expression (Neg/Pos) | Cyclin-D1 Expression | Ki-67 Expression |
---|---|---|---|---|---|---|---|
Age | ρ = 1 | 0.049 | −0.151 | −0.062 | −0.006 | 0.021 | 0.148 |
p-value = 1 | 0.617 | 0.122 | 0.528 | 0.955 | 0.865 | 0.226 | |
Grade | ρ = 1 | 0.098 | 0.034 | 0.223 | 0.060 | 0.398 | |
p-value = 1 | 0.313 | 0.726 | −0.066 | −0.044 | 0.001 * | ||
Stage | ρ = 1 | −0.008 | 0.497 | 0.721 | −0.004 | ||
p-value = 1 | 0.993 | 0.676 | 0.468 | 0.977 | |||
Gender | ρ = 1 | −0.088 | −0.277 | −0.285 | |||
p-value = 1 | 0.364 | 0.023 * | 0.031 * | ||||
MSI2 expression (Neg/Pos) | ρ = 1 | 0.279 | 0.122 | ||||
p-value = 1 | 0.022 * | 0.315 | |||||
Cyclin-D1 expression | ρ = 1 | 0.485 | |||||
p-value = 1 | 0.000 ** | ||||||
Ki-67 expression | ρ = 1 | ||||||
p-value = 1 |
Overall Survival | |||
---|---|---|---|
Variables | Hazard Ratio | 95.0% C.I. | p-Value |
Gender | 1.904 | 0.964–3.759 | 0.064 |
Grade | 1.162 | 0.706–1.911 | 0.555 |
Age | 1.018 | 0.986–1.052 | 0.274 |
Stage | 2.968 | 1.844–4.779 | 0.000 * |
MSI2 (Pos/Neg) | 0.621 | 0.299–1.288 | 0.201 |
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Troiano, G.; Caponio, V.C.A.; Botti, G.; Aquino, G.; Losito, N.S.; Pedicillo, M.C.; Zhurakivska, K.; Arena, C.; Ciavarella, D.; Mastrangelo, F.; et al. Immunohistochemical Analysis Revealed a Correlation between Musashi-2 and Cyclin-D1 Expression in Patients with Oral Squamous Cells Carcinoma. Int. J. Mol. Sci. 2020, 21, 121. https://doi.org/10.3390/ijms21010121
Troiano G, Caponio VCA, Botti G, Aquino G, Losito NS, Pedicillo MC, Zhurakivska K, Arena C, Ciavarella D, Mastrangelo F, et al. Immunohistochemical Analysis Revealed a Correlation between Musashi-2 and Cyclin-D1 Expression in Patients with Oral Squamous Cells Carcinoma. International Journal of Molecular Sciences. 2020; 21(1):121. https://doi.org/10.3390/ijms21010121
Chicago/Turabian StyleTroiano, Giuseppe, Vito Carlo Alberto Caponio, Gerardo Botti, Gabriella Aquino, Nunzia Simona Losito, Maria Carmela Pedicillo, Khrystyna Zhurakivska, Claudia Arena, Domenico Ciavarella, Filiberto Mastrangelo, and et al. 2020. "Immunohistochemical Analysis Revealed a Correlation between Musashi-2 and Cyclin-D1 Expression in Patients with Oral Squamous Cells Carcinoma" International Journal of Molecular Sciences 21, no. 1: 121. https://doi.org/10.3390/ijms21010121
APA StyleTroiano, G., Caponio, V. C. A., Botti, G., Aquino, G., Losito, N. S., Pedicillo, M. C., Zhurakivska, K., Arena, C., Ciavarella, D., Mastrangelo, F., Lo Russo, L., Lo Muzio, L., & Pannone, G. (2020). Immunohistochemical Analysis Revealed a Correlation between Musashi-2 and Cyclin-D1 Expression in Patients with Oral Squamous Cells Carcinoma. International Journal of Molecular Sciences, 21(1), 121. https://doi.org/10.3390/ijms21010121