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Open AccessArticle

Boron Deficiency Increases Cytosolic Ca2+ Levels Mainly via Ca2+ Influx from the Apoplast in Arabidopsis thaliana Roots

Departamento de Fisiología, Anatomía y Biología Celular, Universidad Pablo de Olavide, E-41013 Sevilla, Spain
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Int. J. Mol. Sci. 2019, 20(9), 2297; https://doi.org/10.3390/ijms20092297
Received: 11 April 2019 / Revised: 3 May 2019 / Accepted: 7 May 2019 / Published: 9 May 2019
(This article belongs to the Special Issue Novel Aspects of Boron Biology in Plants. Boron and Plant Interaction)
Boron (B) is a micronutrient for plant development, and its deficiency alters many physiological processes. However, the current knowledge on how plants are able to sense the B-starvation signal is still very limited. Recently, it has been reported that B deprivation induces an increase in cytosolic calcium concentration ([Ca2+]cyt) in Arabidopsis thaliana roots. The aim of this work was to research in Arabidopsis whether [Ca2+]cyt is restored to initial levels when B is resupplied and elucidate whether apoplastic Ca2+ is the major source for B-deficiency-induced rise in [Ca2+]cyt. The use of chemical compounds affecting Ca2+ homeostasis showed that the rise in root [Ca2+]cyt induced by B deficiency was predominantly owed to Ca2+ influx from the apoplast through plasma membrane Ca2+ channels in an IP3-independent manner. Furthermore, B resupply restored the root [Ca2+]cyt. Interestingly, expression levels of genes encoding Ca2+ transporters (ACA10, plasma membrane PIIB-type Ca2+-ATPase; and CAX3, vacuolar cation/proton exchanger) were upregulated by ethylene glycol tetraacetic acid (EGTA) and abscisic acid (ABA). The results pointed out that ACA10, and especially CAX3, would play a major role in the restoration of Ca2+ homeostasis after 24 h of B deficiency. View Full-Text
Keywords: apoplastic calcium; boron deficiency; calcium signaling; cytosolic calcium; Cameleon YC3.6; Arabidopsis thaliana apoplastic calcium; boron deficiency; calcium signaling; cytosolic calcium; Cameleon YC3.6; Arabidopsis thaliana
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Quiles-Pando, C.; Navarro-Gochicoa, M.T.; Herrera-Rodríguez, M.B.; Camacho-Cristóbal, J.J.; González-Fontes, A.; Rexach, J. Boron Deficiency Increases Cytosolic Ca2+ Levels Mainly via Ca2+ Influx from the Apoplast in Arabidopsis thaliana Roots. Int. J. Mol. Sci. 2019, 20, 2297.

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