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Identification of Tea Plant Purple Acid Phosphatase Genes and Their Expression Responses to Excess Iron

Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(8), 1954; https://doi.org/10.3390/ijms20081954
Received: 4 April 2019 / Revised: 19 April 2019 / Accepted: 19 April 2019 / Published: 21 April 2019
(This article belongs to the Section Molecular Plant Sciences)
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Abstract

Purple acid phosphatase (PAP) encoding genes are a multigene family. PAPs require iron (Fe) to exert their functions that are involved in diverse biological roles including Fe homeostasis. However, the possible roles of PAPs in response to excess Fe remain unknown. In this study, we attempted to understand the regulation of PAPs by excess Fe in tea plant (Camellia sinensis). A genome-wide investigation of PAP encoding genes identified 19 CsPAP members based on the conserved motifs. The phylogenetic analysis showed that PAPs could be clustered into four groups, of which group II contained two specific cysteine-containing motifs “GGECGV” and “YERTC”. To explore the expression patterns of CsPAP genes in response to excessive Fe supply, RNA-sequencing (RNA-seq) analyses were performed to compare their transcript abundances between tea plants that are grown under normal and high iron conditions, respectively. 17 members were shown to be transcribed in both roots and leaves. When supplied with a high amount of iron, the expression levels of four genes were significantly changed. Of which, CsPAP15a, CsPAP23 and CsPAP27c were shown as downregulated, while the highly expressed CsPAP10a was upregulated. Moreover, CsPAP23 was found to be alternatively spliced, suggesting its post-transcriptional regulation. The present work implicates that some CsPAP genes could be associated with the responses of tea plants to the iron regime, which may offer a new direction towards a further understanding of iron homeostasis and provide the potential approaches for crop improvement in terms of iron biofortification. View Full-Text
Keywords: PAP; conserved motif; transcriptome; Fe; Camellia sinensis PAP; conserved motif; transcriptome; Fe; Camellia sinensis
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Yin, C.; Wang, F.; Fan, H.; Fang, Y.; Li, W. Identification of Tea Plant Purple Acid Phosphatase Genes and Their Expression Responses to Excess Iron. Int. J. Mol. Sci. 2019, 20, 1954.

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