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Open AccessArticle

Transcriptome Analysis of Larval Segment Formation and Secondary Loss in the Echiuran Worm Urechis unicinctus

College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
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Int. J. Mol. Sci. 2019, 20(8), 1806; https://doi.org/10.3390/ijms20081806
Received: 12 February 2019 / Revised: 23 March 2019 / Accepted: 10 April 2019 / Published: 12 April 2019
(This article belongs to the Section Molecular Genetics and Genomics)
The larval segment formation and secondary loss in echiurans is a special phenomenon, which is considered to be one of the important characteristics in the evolutionary relationship between the Echiura and Annelida. To better understand the molecular mechanism of this phenomenon, we revealed the larval transcriptome profile of the echiuran worm Urechis unicinctus using RNA-Seq technology. Twelve cDNA libraries of U. unicinctus larvae, late-trochophore (LT), early-segmentation larva (ES), segmentation larva (SL), and worm-shaped larva (WL) were constructed. Totally 243,381 unigenes were assembled with an average length of 1125 bp and N50 of 1836 bp, and 149,488 unigenes (61.42%) were annotated. We obtained 70,517 differentially expressed genes (DEGs) by pairwise comparison of the larval transcriptome data at different developmental stages and clustered them into 20 gene expression profiles using STEM software. Based on the typical profiles during the larval segment formation and secondary loss, eight signaling pathways were enriched, and five of which, mTOR, PI3K-AKT, TGF-β, MAPK, and Dorso-ventral axis formation signaling pathway, were proposed for the first time to be involved in the segment formation. Furthermore, we identified 119 unigenes related to the segment formation of annelids, arthropods, and chordates, in which 101 genes were identified in Drosophila and annelids. The function of most segment polarity gene homologs (hedgehog, wingless, engrailed, etc.) was conserved in echiurans, annelids, and arthropods based on their expression profiles, while the gap and pair-rule gene homologs were not. Finally, we verified that strong positive signals of Hedgehog were indeed located on the boundary of larval segments using immunofluorescence. Data in this study provide molecular evidence for the understanding of larval segment development in echiurans and may serve as a blueprint for segmented ancestors in future research. View Full-Text
Keywords: Urechis unicinctus; Echiura; transcriptome; segmentation; Hedgehog pathway Urechis unicinctus; Echiura; transcriptome; segmentation; Hedgehog pathway
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MDPI and ACS Style

Hou, X.; Wei, M.; Li, Q.; Zhang, T.; Zhou, D.; Kong, D.; Xie, Y.; Qin, Z.; Zhang, Z. Transcriptome Analysis of Larval Segment Formation and Secondary Loss in the Echiuran Worm Urechis unicinctus. Int. J. Mol. Sci. 2019, 20, 1806. https://doi.org/10.3390/ijms20081806

AMA Style

Hou X, Wei M, Li Q, Zhang T, Zhou D, Kong D, Xie Y, Qin Z, Zhang Z. Transcriptome Analysis of Larval Segment Formation and Secondary Loss in the Echiuran Worm Urechis unicinctus. International Journal of Molecular Sciences. 2019; 20(8):1806. https://doi.org/10.3390/ijms20081806

Chicago/Turabian Style

Hou, Xitan; Wei, Maokai; Li, Qi; Zhang, Tingting; Zhou, Di; Kong, Dexu; Xie, Yueyang; Qin, Zhenkui; Zhang, Zhifeng. 2019. "Transcriptome Analysis of Larval Segment Formation and Secondary Loss in the Echiuran Worm Urechis unicinctus" Int. J. Mol. Sci. 20, no. 8: 1806. https://doi.org/10.3390/ijms20081806

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