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Article

Horse Oil Mitigates Oxidative Damage to Human HaCaT Keratinocytes Caused by Ultraviolet B Irradiation

1
School of Medicine, Jeju National University, Jeju 63243, Korea
2
Bio Convergence Center, Jeju Technopark, Jeju 63243, Korea
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(6), 1490; https://doi.org/10.3390/ijms20061490
Received: 14 February 2019 / Revised: 18 March 2019 / Accepted: 22 March 2019 / Published: 25 March 2019
(This article belongs to the Section Biochemistry)
Horse oil products have been used in skin care for a long time in traditional medicine, but the biological effects of horse oil on the skin remain unclear. This study was conducted to evaluate the protective effect of horse oil on ultraviolet B (UVB)-induced oxidative stress in human HaCaT keratinocytes. Horse oil significantly reduced UVB-induced intracellular reactive oxygen species and intracellular oxidative damage to lipids, proteins, and DNA. Horse oil absorbed light in the UVB range of the electromagnetic spectrum and suppressed the generation of cyclobutane pyrimidine dimers, a photoproduct of UVB irradiation. Western blotting showed that horse oil increased the UVB-induced Bcl-2/Bax ratio, inhibited mitochondria-mediated apoptosis and matrix metalloproteinase expression, and altered mitogen-activated protein kinase signaling-related proteins. These effects were conferred by increased phosphorylation of extracellular signal-regulated kinase 1/2 and decreased phosphorylation of p38 and c-Jun N-terminal kinase 1/2. Additionally, horse oil reduced UVB-induced binding of activator protein 1 to the matrix metalloproteinase-1 promoter site. These results indicate that horse oil protects human HaCaT keratinocytes from UVB-induced oxidative stress by absorbing UVB radiation and removing reactive oxygen species, thereby protecting cells from structural damage and preventing cell death and aging. In conclusion, horse oil is a potential skin protectant against skin damage involving oxidative stress. View Full-Text
Keywords: horse oil; ultraviolet B radiation; oxidative stress; apoptosis horse oil; ultraviolet B radiation; oxidative stress; apoptosis
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MDPI and ACS Style

Piao, M.J.; Kang, K.A.; Zhen, A.X.; Kang, H.K.; Koh, Y.S.; Kim, B.S.; Hyun, J.W. Horse Oil Mitigates Oxidative Damage to Human HaCaT Keratinocytes Caused by Ultraviolet B Irradiation. Int. J. Mol. Sci. 2019, 20, 1490. https://doi.org/10.3390/ijms20061490

AMA Style

Piao MJ, Kang KA, Zhen AX, Kang HK, Koh YS, Kim BS, Hyun JW. Horse Oil Mitigates Oxidative Damage to Human HaCaT Keratinocytes Caused by Ultraviolet B Irradiation. International Journal of Molecular Sciences. 2019; 20(6):1490. https://doi.org/10.3390/ijms20061490

Chicago/Turabian Style

Piao, Mei J., Kyoung A. Kang, Ao X. Zhen, Hee K. Kang, Young S. Koh, Bong S. Kim, and Jin W. Hyun 2019. "Horse Oil Mitigates Oxidative Damage to Human HaCaT Keratinocytes Caused by Ultraviolet B Irradiation" International Journal of Molecular Sciences 20, no. 6: 1490. https://doi.org/10.3390/ijms20061490

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