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Article

Peanut Sprout Extracts Attenuate Triglyceride Accumulation by Promoting Mitochondrial Fatty Acid Oxidation in Adipocytes

1
Department of Marine Life Science, Jeju National University, Jeju 63243, Korea
2
Department of Food Science and Nutrition, Jeju National University, Jeju 63243, Korea
3
Department of Food and Nutrition, Kyungnam College of Information & Technology, Pusan 47011, Korea
4
Department of Food and Nutrition, Sungshin Women’s University, Seoul 01133, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(5), 1216; https://doi.org/10.3390/ijms20051216
Received: 13 February 2019 / Revised: 5 March 2019 / Accepted: 6 March 2019 / Published: 11 March 2019
(This article belongs to the Special Issue Health Promoting Effects of Phytochemicals)
Peanut sprouts (PS), which are germinated peanut seeds, have recently been reported to have anti-oxidant, anti-inflammatory, and anti-obesity effects. However, the underlying mechanisms by which PS modulates lipid metabolism are largely unknown. To address this question, serial doses of PS extract (PSE) were added to 3T3-L1 cells during adipocyte differentiation. PSE (25 µg/mL) significantly attenuated adipogenesis by inhibiting lipid accumulation in addition to reducing the level of adipogenic protein and gene expression with the activation of AMP-activated protein kinase (AMPK). Other adipocyte cell models such as mouse embryonic fibroblasts C3H10T1/2 and primary adipocytes also confirmed the anti-adipogenic properties of PSE. Next, we investigated whether PSE attenuated lipid accumulation in mature adipocytes. We found that PSE significantly suppressed lipogenic gene expression, while fatty acid (FA) oxidation genes were upregulated. Augmentation of FA oxidation by PSE in mature 3T3-L1 adipocytes was confirmed via a radiolabeled-FA oxidation rate experiment by measuring the conversion of [3H]-oleic acid (OA) to [3H]-H2O. Furthermore, PSE enhanced the mitochondrial oxygen consumption rate (OCR), especially maximal respiration, and beige adipocyte formation in adipocytes. In summary, PSE was effective in reducing lipid accumulation in 3T3-L1 adipocytes through mitochondrial fatty acid oxidation involved in AMPK and mitochondrial activation. View Full-Text
Keywords: peanut sprouts; resveratrol; adipogenesis; fatty acid oxidation; mitochondrial respiration peanut sprouts; resveratrol; adipogenesis; fatty acid oxidation; mitochondrial respiration
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MDPI and ACS Style

Seo, S.H.; Jo, S.-M.; Kim, J.; Lee, M.; Lee, Y.; Kang, I. Peanut Sprout Extracts Attenuate Triglyceride Accumulation by Promoting Mitochondrial Fatty Acid Oxidation in Adipocytes. Int. J. Mol. Sci. 2019, 20, 1216. https://doi.org/10.3390/ijms20051216

AMA Style

Seo SH, Jo S-M, Kim J, Lee M, Lee Y, Kang I. Peanut Sprout Extracts Attenuate Triglyceride Accumulation by Promoting Mitochondrial Fatty Acid Oxidation in Adipocytes. International Journal of Molecular Sciences. 2019; 20(5):1216. https://doi.org/10.3390/ijms20051216

Chicago/Turabian Style

Seo, Seok H., Sang-Mi Jo, Jiyoung Kim, Myoungsook Lee, Yunkyoung Lee, and Inhae Kang. 2019. "Peanut Sprout Extracts Attenuate Triglyceride Accumulation by Promoting Mitochondrial Fatty Acid Oxidation in Adipocytes" International Journal of Molecular Sciences 20, no. 5: 1216. https://doi.org/10.3390/ijms20051216

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