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Open AccessArticle

An Anabolic Signaling Response of Rat Soleus Muscle to Eccentric Contractions Following Hindlimb Unloading: A Potential Role of Stretch-Activated Ion Channels

Myology Laboratory, Institute of Biomedical Problems RAS, 123007, 76A Khoroshevskoe shosse, 123007 Moscow, Russia
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Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(5), 1165; https://doi.org/10.3390/ijms20051165
Received: 8 February 2019 / Revised: 2 March 2019 / Accepted: 4 March 2019 / Published: 7 March 2019
(This article belongs to the Special Issue Kinase Signal Transduction 2019)
Mechanisms that convert a mechanical signal into a biochemical response in an atrophied skeletal muscle remain poorly understood. The aims of the study were to evaluate a temporal response of anabolic signaling and protein synthesis (PS) to eccentric contractions (EC) in rat soleus during hindlimb unloading (HU); and to assess a possible role of stretch-activated ion channels (SAC) in the propagation of a mechanical signal to mTORC1 following HU. Following HU, an isolated soleus was subjected to EC. Upon completion of EC, muscles were collected for western blot analyses to determine the content/phosphorylation of the key anabolic markers. We found that a degree of EC-induced p70S6K phosphorylation and the rate of PS in the soleus of 3- and 7-day unloaded rats was significantly less than that in control. A decrease in EC-induced phosphorylation of p70S6K, RPS6 and PS in the 7-day unloaded soleus treated with SAC inhibitor did not differ from that of the 7-day unloaded soleus without SAC blockade. The results of the study suggest that (i) HU results in a blunted anabolic response to a bout of EC, (ii) attenuation of mTORC1-signaling and PS in response to EC in unloaded soleus may be associated with inactivation of SAC. View Full-Text
Keywords: soleus muscle; hindlimb unloading; eccentric contractions; protein synthesis; anabolic signaling; stretch-activated channels soleus muscle; hindlimb unloading; eccentric contractions; protein synthesis; anabolic signaling; stretch-activated channels
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Tyganov, S.; Mirzoev, T.; Shenkman, B. An Anabolic Signaling Response of Rat Soleus Muscle to Eccentric Contractions Following Hindlimb Unloading: A Potential Role of Stretch-Activated Ion Channels. Int. J. Mol. Sci. 2019, 20, 1165.

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