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Open AccessArticle

On a Cold Night: Transcriptomics of Grapevine Flower Unveils Signal Transduction and Impacted Metabolism

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Unité de Recherche Résistance Induite et Bioprotection des Plantes—EA 4707, Université de Reims Champagne-Ardenne, UFR Sciences Exactes et Naturelles, SFR Condorcet FR CNRS 3417, Moulin de la Housse—Bâtiment 18, BP 1039, 51687 REIMS Cedex 2, France
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Luxembourg Institute of Science and Technology (LIST), Environmental Research and Innovation (ERIN) Department, 41 rue du Brill, L- 4422 Belvaux, Luxembourg
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Institute of Vine and Wine Sciences, UMR 1287 Ecophysiology and Grape Functional Genomics, University of Bordeaux, INRA 210 Chemin de Leysotte - CS 50008, 33882 Villenave d’Ornon CEDEX, France
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Institute of Plant Sciences Paris Saclay IPS2, CNRS, INRA, Université Paris-Sud, Université Evry, Université Paris-Saclay, Bâtiment 630, 91405 Orsay, France
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Institute of Plant Sciences Paris-Saclay IPS2, Paris Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405, Orsay, France
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IRHS, INRA, AGROCAMPUS-Ouest, Université d’Angers, SFR 4207 QUASAV, 42 rue Georges Morel, 49071 Beaucouzé CEDEX, France
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(5), 1130; https://doi.org/10.3390/ijms20051130
Received: 11 February 2019 / Revised: 26 February 2019 / Accepted: 1 March 2019 / Published: 5 March 2019
(This article belongs to the Special Issue Plant Metabolism in Crops: A Systems Biology Perspective)
Low temperature is a critical environmental factor limiting plant productivity, especially in northern vineyards. To clarify the impact of this stress on grapevine flower, we used the Vitis array based on Roche-NimbleGen technology to investigate the gene expression of flowers submitted to a cold night. Our objectives were to identify modifications in the transcript levels after stress and during recovery. Consequently, our results confirmed some mechanisms known in grapes or other plants in response to cold stress, notably, (1) the pivotal role of calcium/calmodulin-mediated signaling; (2) the over-expression of sugar transporters and some genes involved in plant defense (especially in carbon metabolism), and (3) the down-regulation of genes encoding galactinol synthase (GOLS), pectate lyases, or polygalacturonases. We also identified some mechanisms not yet known to be involved in the response to cold stress, i.e., (1) the up-regulation of genes encoding G-type lectin S-receptor-like serine threonine-protein kinase, pathogen recognition receptor (PRR5), or heat-shock factors among others; (2) the down-regulation of Myeloblastosis (MYB)-related transcription factors and the Constans-like zinc finger family; and (3) the down-regulation of some genes encoding Pathogen-Related (PR)-proteins. Taken together, our results revealed interesting features and potentially valuable traits associated with stress responses in the grapevine flower. From a long-term perspective, our study provides useful starting points for future investigation. View Full-Text
Keywords: Vitis vinifera; flower; candidate genes expression; cold stress; signaling cascades; secondary metabolism; cell wall Vitis vinifera; flower; candidate genes expression; cold stress; signaling cascades; secondary metabolism; cell wall
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Sawicki, M.; Rondeau, M.; Courteaux, B.; Rabenoelina, F.; Guerriero, G.; Gomès, E.; Soubigou-Taconnat, L.; Balzergue, S.; Clément, C.; Ait Barka, E.; Vaillant-Gaveau, N.; Jacquard, C. On a Cold Night: Transcriptomics of Grapevine Flower Unveils Signal Transduction and Impacted Metabolism. Int. J. Mol. Sci. 2019, 20, 1130.

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