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Open AccessArticle

Bioproduction of Quercetin and Rutinose Catalyzed by Rutinosidase: Novel Concept of “Solid State Biocatalysis”

Institute of Microbiology of the Czech Academy of Sciences, Laboratory of Biotransformation, Vídeňská 1083, CZ 14220 Prague 4, Czech Republic
Institute of Biotechnology, Slovak University of Technology, Radlinského 9, 81237 Bratislava, Slovakia
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(5), 1112;
Received: 7 February 2019 / Revised: 25 February 2019 / Accepted: 28 February 2019 / Published: 5 March 2019
(This article belongs to the Special Issue Molecular Biocatalysis 2.0)
Quercetin is a flavonoid largely employed as a phytochemical remedy and a food or dietary supplement. We present here a novel biocatalytic methodology for the preparation of quercetin from plant-derived rutin, with both substrate and product being in mostly an undissolved state during biotransformation. This “solid-state” enzymatic conversion uses a crude enzyme preparation of recombinant rutinosidase from Aspergillus niger yielding quercetin, which precipitates from virtually insoluble rutin. The process is easily scalable and exhibits an extremely high space-time yield. The procedure has been shown to be robust and was successfully tested with rutin concentrations of up to 300 g/L (ca 0.5 M) at various scales. Using this procedure, pure quercetin is easily obtained by mere filtration of the reaction mixture, followed by washing and drying of the filter cake. Neither co-solvents nor toxic chemicals are used, thus the process can be considered environmentally friendly and the product of “bio-quality.” Moreover, rare disaccharide rutinose is obtained from the filtrate at a preparatory scale as a valuable side product. These results demonstrate for the first time the efficiency of the “Solid-State-Catalysis” concept, which is applicable virtually for any biotransformation involving substrates and products of low water solubility. View Full-Text
Keywords: Aspergillus niger; quercetin; rutin; rutinose; rutinosidase; “solid-state biocatalysis” Aspergillus niger; quercetin; rutin; rutinose; rutinosidase; “solid-state biocatalysis”
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MDPI and ACS Style

Kapešová, J.; Petrásková, L.; Markošová, K.; Rebroš, M.; Kotik, M.; Bojarová, P.; Křen, V. Bioproduction of Quercetin and Rutinose Catalyzed by Rutinosidase: Novel Concept of “Solid State Biocatalysis”. Int. J. Mol. Sci. 2019, 20, 1112.

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