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Open AccessArticle

Bioproduction of Quercetin and Rutinose Catalyzed by Rutinosidase: Novel Concept of “Solid State Biocatalysis”

1
Institute of Microbiology of the Czech Academy of Sciences, Laboratory of Biotransformation, Vídeňská 1083, CZ 14220 Prague 4, Czech Republic
2
Institute of Biotechnology, Slovak University of Technology, Radlinského 9, 81237 Bratislava, Slovakia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(5), 1112; https://doi.org/10.3390/ijms20051112
Received: 7 February 2019 / Revised: 25 February 2019 / Accepted: 28 February 2019 / Published: 5 March 2019
(This article belongs to the Special Issue Molecular Biocatalysis 2.0)
Quercetin is a flavonoid largely employed as a phytochemical remedy and a food or dietary supplement. We present here a novel biocatalytic methodology for the preparation of quercetin from plant-derived rutin, with both substrate and product being in mostly an undissolved state during biotransformation. This “solid-state” enzymatic conversion uses a crude enzyme preparation of recombinant rutinosidase from Aspergillus niger yielding quercetin, which precipitates from virtually insoluble rutin. The process is easily scalable and exhibits an extremely high space-time yield. The procedure has been shown to be robust and was successfully tested with rutin concentrations of up to 300 g/L (ca 0.5 M) at various scales. Using this procedure, pure quercetin is easily obtained by mere filtration of the reaction mixture, followed by washing and drying of the filter cake. Neither co-solvents nor toxic chemicals are used, thus the process can be considered environmentally friendly and the product of “bio-quality.” Moreover, rare disaccharide rutinose is obtained from the filtrate at a preparatory scale as a valuable side product. These results demonstrate for the first time the efficiency of the “Solid-State-Catalysis” concept, which is applicable virtually for any biotransformation involving substrates and products of low water solubility. View Full-Text
Keywords: Aspergillus niger; quercetin; rutin; rutinose; rutinosidase; “solid-state biocatalysis” Aspergillus niger; quercetin; rutin; rutinose; rutinosidase; “solid-state biocatalysis”
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MDPI and ACS Style

Kapešová, J.; Petrásková, L.; Markošová, K.; Rebroš, M.; Kotik, M.; Bojarová, P.; Křen, V. Bioproduction of Quercetin and Rutinose Catalyzed by Rutinosidase: Novel Concept of “Solid State Biocatalysis”. Int. J. Mol. Sci. 2019, 20, 1112.

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