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Article

Platelet-Rich Fibrin Extract: A Promising Fetal Bovine Serum Alternative in Explant Cultures of Human Periosteal Sheets for Regenerative Therapy

1
Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan
2
Division of Oral Surgery, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan
3
Division of Periodontology, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan
4
Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata 951-8520, Japan
5
Kohjin Bio Co., Ltd., Sakado 350-0214, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(5), 1053; https://doi.org/10.3390/ijms20051053
Received: 24 January 2019 / Revised: 25 February 2019 / Accepted: 25 February 2019 / Published: 28 February 2019
(This article belongs to the Special Issue Blood-Derived Products for Tissue Repair/Regeneration)
In 2004, we developed autologous periosteal sheets for the treatment of periodontal bone defects. This regenerative therapy has successfully regenerated periodontal bone and augmented alveolar ridge for implant placement. However, the necessity for 6-week culture is a limitation. Here, we examined the applicability of a human platelet-rich fibrin extract (PRFext) as an alternative to fetal bovine serum (FBS) for the explant culture of periosteal sheets in a novel culture medium (MSC-PCM) originally developed for maintaining mesenchymal stem cells. Small periosteum tissue segments were expanded in MSC-PCM + 2% PRFext for 4 weeks, and the resulting periosteal sheets were compared with those prepared by the conventional method using Medium199 + 10% FBS for their growth rate, cell multilayer formation, alkaline phosphatase (ALP) activity, and surface antigen expression (CD73, CD90, and CD105). Periosteal sheets grew faster in the novel culture medium than in the conventional medium. However, assessment of cell shape and ALP activity revealed that the periosteal cells growing in the novel medium were relatively immature. These findings suggest that the novel culture medium featuring PRFext offers advantages by shortening the culture period and excluding possible risks associated with xeno-factors without negatively altering the activity of periosteal sheets. View Full-Text
Keywords: periosteal sheet; platelet-rich fibrin; growth; differentiation; bone grafting material periosteal sheet; platelet-rich fibrin; growth; differentiation; bone grafting material
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MDPI and ACS Style

Kawase, T.; Nagata, M.; Okuda, K.; Ushiki, T.; Fujimoto, Y.; Watanabe, M.; Ito, A.; Nakata, K. Platelet-Rich Fibrin Extract: A Promising Fetal Bovine Serum Alternative in Explant Cultures of Human Periosteal Sheets for Regenerative Therapy. Int. J. Mol. Sci. 2019, 20, 1053. https://doi.org/10.3390/ijms20051053

AMA Style

Kawase T, Nagata M, Okuda K, Ushiki T, Fujimoto Y, Watanabe M, Ito A, Nakata K. Platelet-Rich Fibrin Extract: A Promising Fetal Bovine Serum Alternative in Explant Cultures of Human Periosteal Sheets for Regenerative Therapy. International Journal of Molecular Sciences. 2019; 20(5):1053. https://doi.org/10.3390/ijms20051053

Chicago/Turabian Style

Kawase, Tomoyuki, Masaki Nagata, Kazuhiro Okuda, Takashi Ushiki, Yoko Fujimoto, Mari Watanabe, Akira Ito, and Koh Nakata. 2019. "Platelet-Rich Fibrin Extract: A Promising Fetal Bovine Serum Alternative in Explant Cultures of Human Periosteal Sheets for Regenerative Therapy" International Journal of Molecular Sciences 20, no. 5: 1053. https://doi.org/10.3390/ijms20051053

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