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Open AccessArticle

Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies

1
Unité de Génie Enzymatique et Cellulaire, CNRS UMR 7025, Université de Picardie Jules Verne (UPJV), 80039 Amiens, France
2
Unité de Génie Enzymatique et Cellulaire, CNRS UMR 7025, Sorbonne Universités, Université de Technologie de Compiègne, 60200 Compiègne, France
3
Laboratoire de Biophysique Moléculaire aux Interfaces, Gembloux Agro-Bio Tech, B5030 Gembloux, Belgium
4
Matrice Extracellulaire et Dynamique Cellulaire, UMR CNRS 7369, Chaire MAgICS, Université de Reims Champagne-Ardenne (URCA), 51687 Reims, France
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(5), 1009; https://doi.org/10.3390/ijms20051009
Received: 30 January 2019 / Revised: 20 February 2019 / Accepted: 23 February 2019 / Published: 26 February 2019
Rhamnolipids (RLs) are potential biocontrol agents for crop culture protection. Their mode of action has been proposed as dual, combining plant protection activation and antifungal activities. The present work focuses on the interaction of natural RLs with plant and fungi membrane models at the molecular scale. Representative models were constructed and the interaction with RLs was studied by Fourier transform infrared (FTIR) and deuterium nuclear magnetic resonance (2H NMR) spectroscopic measurements. Molecular dynamic (MD) simulations were performed to investigate RL insertion in lipid bilayers. Our results showed that the RLs fit into the membrane models and were located near the lipid phosphate group of the phospholipid bilayers, nearby phospholipid glycerol backbones. The results obtained with plant plasma membrane models suggest that the insertion of RLs inside the lipid bilayer did not significantly affect lipid dynamics. Oppositely, a clear fluidity increase of fungi membrane models was observed. This effect was related to the presence and the specific structure of ergosterol. The nature of the phytosterols could also influence the RL effect on plant plasma membrane destabilization. Subtle changes in lipid dynamics could then be linked with plant defense induction and the more drastic effects associated with fungal membrane destabilization. View Full-Text
Keywords: rhamnolipid; membrane insertion and dynamic; biomimetic lipid membrane; plant plasma membrane; fungi plasma membrane; membrane sterol; phytosterols; ergosterol rhamnolipid; membrane insertion and dynamic; biomimetic lipid membrane; plant plasma membrane; fungi plasma membrane; membrane sterol; phytosterols; ergosterol
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MDPI and ACS Style

Monnier, N.; Furlan, A.L.; Buchoux, S.; Deleu, M.; Dauchez, M.; Rippa, S.; Sarazin, C. Exploring the Dual Interaction of Natural Rhamnolipids with Plant and Fungal Biomimetic Plasma Membranes through Biophysical Studies. Int. J. Mol. Sci. 2019, 20, 1009.

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