Translational Control using an Expanded Genetic Code
Abstract
:1. Introduction
2. Classifications
3. G1
3.1. Thermolabile tRNACUA
3.2. Inducible Transcription of tRNACUA
3.3. Inducible Aminoacyl-tRNA Synthetase
3.4. Introduction of the tRNACUA Gene
4. G2
5. G3
5.1. Inducible UaaRS/tRNACUA
5.2. Optimization of the UaaRS/tRNACUA Expression Level
5.3. Multiplexed TAG Insertion in a Target Gene
5.4. Location of the UAG Insertion
5.5. Uaa-Residue Conversion to Standard Amino Acid Residue
5.6. Suppression of Peptide Release Factor
5.7. Uaa-Permissive Elongation Factor
5.8. Host Selection
5.9. Improved UaaRS/tRNACUA
6. Uaa Transcriptional Switch
7. Applications
7.1. Biological Containment
7.2. Live Attenuated-Virus Vaccine
7.3. High-yield and Zero-Leakage Expression System (HYZEL)
8. Conclusions
Funding
Conflicts of Interest
References
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Kato, Y. Translational Control using an Expanded Genetic Code. Int. J. Mol. Sci. 2019, 20, 887. https://doi.org/10.3390/ijms20040887
Kato Y. Translational Control using an Expanded Genetic Code. International Journal of Molecular Sciences. 2019; 20(4):887. https://doi.org/10.3390/ijms20040887
Chicago/Turabian StyleKato, Yusuke. 2019. "Translational Control using an Expanded Genetic Code" International Journal of Molecular Sciences 20, no. 4: 887. https://doi.org/10.3390/ijms20040887