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Int. J. Mol. Sci. 2019, 20(3), 761; https://doi.org/10.3390/ijms20030761

Transcriptome Analysis Reveals New Insights into the Bacterial Wilt Resistance Mechanism Mediated by Silicon in Tomato

1
College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China
2
Institute of Tropical and Subtropical Ecology, South China Agricultural University, Guangzhou 510642, China
3
Tea Research Institute, Guangdong Academy of Agricultural Science/Guangdong Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou 510640, China
4
College of Horticulture, South China Agricultural University, Guangzhou 510642, China
*
Author to whom correspondence should be addressed.
Received: 8 January 2019 / Revised: 30 January 2019 / Accepted: 4 February 2019 / Published: 11 February 2019
(This article belongs to the Special Issue Plant Microbe Interaction 2019)
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Abstract

Bacterial wilt is a devastating disease of tomato caused by soilborne pathogenic bacterium Ralstonia solanacearum. Previous studies found that silicon (Si) can increase tomato resistance against R. solanacearum, but the exact molecular mechanism remains unclear. RNA sequencing (RNA-Seq) technology was used to investigate the dynamic changes of root transcriptome profiles between Si-treated (+Si) and untreated (−Si) tomato plants at 1, 3, and 7 days post-inoculation with R. solanacearum. The contents of salicylic acid (SA), ethylene (ET), and jasmonic acid (JA) and the activity of defense-related enzymes in roots of tomato in different treatments were also determined. The burst of ET production in roots was delayed, and SA and JA contents were altered in Si treatment. The transcriptional response to R. solanacearum infection of the +Si plants was quicker than that of the untreated plants. The expression levels of differentially-expressed genes involved in pathogen-associated molecular pattern-triggered immunity (PTI), oxidation resistance, and water-deficit stress tolerance were upregulated in the Si-treated plants. Multiple hormone-related genes were differentially expressed in the Si-treated plants. Si-mediated resistance involves mechanisms other than SA- and JA/ET-mediated stress responses. We propose that Si-mediated tomato resistance to R. solanacearum is associated with activated PTI-related responses and enhanced disease resistance and tolerance via several signaling pathways. Such pathways are mediated by multiple hormones (e.g., SA, JA, ET, and auxin), leading to diminished adverse effects (e.g., senescence, water-deficit, salinity and oxidative stress) normally caused by R. solanacearum infection. This finding will provide an important basis to further characterize the role of Si in enhancing plant resistance against biotic stress. View Full-Text
Keywords: silicon; induced resistance; Ralstonia solanacearum; tomato; transcriptomics silicon; induced resistance; Ralstonia solanacearum; tomato; transcriptomics
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Jiang, N.; Fan, X.; Lin, W.; Wang, G.; Cai, K. Transcriptome Analysis Reveals New Insights into the Bacterial Wilt Resistance Mechanism Mediated by Silicon in Tomato. Int. J. Mol. Sci. 2019, 20, 761.

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