Int. J. Mol. Sci. 2019, 20(3), 758; https://doi.org/10.3390/ijms20030758
Map-Based Cloning and Functional Analysis of YE1 in Rice, Which Is Involved in Light-Dependent Chlorophyll Biogenesis and Photoperiodic Flowering Pathway
Youlin Peng 1,†
, Ting Zou 1,†,*
, Lamei Li 1, Shiwen Tang 1, Qiao Li 2, Jie Zhang 1, Yongjun Chen 1, Xuechun Wang 1, Guotao Yang 1 and Yungao Hu 1,*
, Ting Zou 1,†,*
, Lamei Li 1, Shiwen Tang 1, Qiao Li 2, Jie Zhang 1, Yongjun Chen 1, Xuechun Wang 1, Guotao Yang 1 and Yungao Hu 1,*
1
Rice Research Institute, Southwest University of Science and Technology, Mianyang 621010, China
2
College of Landscape Architectural, Sichuan Agricultural University, Chengdu 611130, China
†
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 12 January 2019 / Revised: 4 February 2019 / Accepted: 7 February 2019 / Published: 11 February 2019
(This article belongs to the Section Molecular Plant Sciences)
Abstract
Light is one of the most important environmental factors that affect many aspects of plant growth, including chlorophyll (Chl) synthesis and flowering time. Here, we identified a rice mutant, yellow leaf and early flowering (ye1), and characterized the gene YE1 by using a map-based cloning method. YE1 encodes a heme oxygenase, which is localized to the chloroplasts. YE1 is expressed in various green tissues, especially in leaves, with a diurnal-rhythmic expression pattern, and its transcripts is also induced by light during leaf-greening. The mutant displays decreased Chl contents with less and disorderly thylakoid lamellar layers in chloroplasts, which reduced the photosynthesis rate. The early flowering phenotype of ye1 was not photoperiod-sensitive. Furthermore, the expression levels of Chl biosynthetic genes were downregulated in ye1 seedlings during de-etiolation responses to light. We also found that rhythmic expression patterns of genes involved in photoperiodic flowering were altered in the mutant. Based on these results, we infer that YE1 plays an important role in light-dependent Chl biogenesis as well as photoperiodic flowering pathway in rice. View Full-TextKeywords:
rice (Oryza sativa L.); light; chlorophyll biosynthesis; photoperiodic flowering; map-based cloning
▼
Figures
Graphical abstract
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
Share & Cite This Article
MDPI and ACS Style
Peng, Y.; Zou, T.; Li, L.; Tang, S.; Li, Q.; Zhang, J.; Chen, Y.; Wang, X.; Yang, G.; Hu, Y. Map-Based Cloning and Functional Analysis of YE1 in Rice, Which Is Involved in Light-Dependent Chlorophyll Biogenesis and Photoperiodic Flowering Pathway. Int. J. Mol. Sci. 2019, 20, 758.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
Related Articles
Article Metrics
Comments
[Return to top]
Int. J. Mol. Sci.
EISSN 1422-0067
Published by MDPI AG, Basel, Switzerland
RSS
E-Mail Table of Contents Alert