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Open AccessArticle

Transcriptional Profile of Aedes aegypti Leucine-Rich Repeat Proteins in Response to Zika and Chikungunya Viruses

Florida Medical Entomology Laboratory, University of Florida, 200 9th Street South East, Vero Beach, FL 32962, USA
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Int. J. Mol. Sci. 2019, 20(3), 615; https://doi.org/10.3390/ijms20030615
Received: 30 December 2018 / Revised: 26 January 2019 / Accepted: 28 January 2019 / Published: 31 January 2019
(This article belongs to the Special Issue Molecular Research on Emerging Mosquito-Transmitted RNA Viruses)
Aedes aegypti (L.) is the primary vector of chikungunya, dengue, yellow fever, and Zika viruses. The leucine-rich repeats (LRR)-containing domain is evolutionarily conserved in many proteins associated with innate immunity in invertebrates and vertebrates, as well as plants. We focused on the AaeLRIM1 and AaeAPL1 gene expressions in response to Zika virus (ZIKV) and chikungunya virus (CHIKV) infection using a time course study, as well as the developmental expressions in the eggs, larvae, pupae, and adults. RNA-seq analysis data provided 60 leucine-rich repeat related transcriptions in Ae. aegypti in response to Zika virus (Accession number: GSE118858, accessed on: August 22, 2018, GEO DataSets). RNA-seq analysis data showed that AaeLRIM1 (AAEL012086-RA) and AaeAPL1 (AAEL009520-RA) were significantly upregulated 2.5 and 3-fold during infection by ZIKV 7-days post infection (dpi) of an Ae. aegypti Key West strain compared to an Orlando strain. The qPCR data showed that LRR-containing proteins related genes, AaeLRIM1 and AaeAPL1, and five paralogues were expressed 100-fold lower than other nuclear genes, such as defensin, during all developmental stages examined. Together, these data provide insights into the transcription profiles of LRR proteins of Ae. aegypti during its development and in response to infection with emergent arboviruses. View Full-Text
Keywords: Aedes aegypti; Leucine-Rich Repeat Proteins; Zika virus; chikungunya virus; immune responses; gene expression Aedes aegypti; Leucine-Rich Repeat Proteins; Zika virus; chikungunya virus; immune responses; gene expression
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MDPI and ACS Style

Zhao, L.; Alto, B.W.; Shin, D. Transcriptional Profile of Aedes aegypti Leucine-Rich Repeat Proteins in Response to Zika and Chikungunya Viruses. Int. J. Mol. Sci. 2019, 20, 615. https://doi.org/10.3390/ijms20030615

AMA Style

Zhao L, Alto BW, Shin D. Transcriptional Profile of Aedes aegypti Leucine-Rich Repeat Proteins in Response to Zika and Chikungunya Viruses. International Journal of Molecular Sciences. 2019; 20(3):615. https://doi.org/10.3390/ijms20030615

Chicago/Turabian Style

Zhao, Liming; Alto, Barry W.; Shin, Dongyoung. 2019. "Transcriptional Profile of Aedes aegypti Leucine-Rich Repeat Proteins in Response to Zika and Chikungunya Viruses" Int. J. Mol. Sci. 20, no. 3: 615. https://doi.org/10.3390/ijms20030615

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