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Open AccessArticle

α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload

1
Department of Biomedical and Biotechnological Sciences, University of Catania, 95123 Catania, Italy
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EuroMediterranean Institute of Science and Technology, 90139 Palermo, Italy
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Department of Medical and Surgical Specialties, Hematology Section, University of Catania, 95125 Catania, Italy
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Department of Biological, Geological and Environmental Science, University of Catania, 95129 Catania, Italy
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Section of Human Anatomy, School of Medicinal and Health Products Sciences, University of Camerino, Via Madonna delle Carceri 9, 62032 Camerino, Italy
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Department of Medical, Surgical Sciences and Advanced Technologies “GF Ingrassia”, University of Catania, 95123 Catania, Italy
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Experimental Zooprophylactic Institute of Sicily, 95125 Catania, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(3), 609; https://doi.org/10.3390/ijms20030609
Received: 8 January 2019 / Revised: 22 January 2019 / Accepted: 24 January 2019 / Published: 31 January 2019
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Iron toxicity is associated with organ injury and has been reported in various clinical conditions, such as hemochromatosis, thalassemia major, and myelodysplastic syndromes. Therefore, iron chelation therapy represents a pivotal therapy for these patients during their lifetime. The aim of the present study was to assess the iron chelating properties of α-lipoic acid (ALA) and how such an effect impacts on iron overload mediated toxicity. Human mesenchymal stem cells (HS-5) and animals (zebrafish, n = 10 for each group) were treated for 24 h with ferric ammonium citrate (FAC, 120 µg/mL) in the presence or absence of ALA (20 µg/mL). Oxidative stress was evaluated by reduced glutathione content, reactive oxygen species formation, mitochondrial dysfunction, and gene expression of heme oxygenase-1b and mitochondrial superoxide dismutase; organ injury, iron accumulation, and autophagy were measured by microscopical, cytofluorimetric analyses, and inductively coupled plasma‒optical mission Spectrometer (ICP-OES). Our results showed that FAC results in a significant increase of tissue iron accumulation, oxidative stress, and autophagy and such detrimental effects were reversed by ALA treatment. In conclusion, ALA possesses excellent iron chelating properties that may be exploited in a clinical setting for organ preservation, as well as exhibiting a good safety profile and low cost for the national health system. View Full-Text
Keywords: iron; alpha lipoic acid; oxidative stress; toxicity; zebrafish iron; alpha lipoic acid; oxidative stress; toxicity; zebrafish
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Camiolo, G.; Tibullo, D.; Giallongo, C.; Romano, A.; Parrinello, N.L.; Musumeci, G.; Di Rosa, M.; Vicario, N.; Brundo, M.V.; Amenta, F.; Ferrante, M.; Copat, C.; Avola, R.; Li Volti, G.; Salvaggio, A.; Di Raimondo, F.; Palumbo, G.A. α-Lipoic Acid Reduces Iron-induced Toxicity and Oxidative Stress in a Model of Iron Overload. Int. J. Mol. Sci. 2019, 20, 609.

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