Forsythoside A and Forsythoside B Contribute to Shuanghuanglian Injection-Induced Pseudoallergic Reactions through the RhoA/ROCK Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. Forsythoside A and Forsythoside B Induce Vascular Leakage in Mice
2.2. Forsythoside A and Forsythoside B Slightly Evoke Mast Cell Degranulation
2.3. Forsythoside A and Forsythoside B Enhance Endothelial Permeability via Activation of the RhoA/ROCK Signaling Pathway
2.4. Fasudil Attenuates the Forsythoside A/Forsythoside B-induced Endothelial Monolayer and Vascular Hyperpermeability
2.5. Docking Analysis of GTP-RhoA with Forsythoside A, Forsythoside B, and Forsythoside E
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Reagents
4.3. Animals
4.4. Cell Culture
4.5. Assessment of Vascular Leakage in Mice
4.6. Histamine Assay
4.7. Assessment of Mast Cell Degranulation
4.8. Endothelial Permeability Assay
4.9. Preparation of Cell Lysates
4.10. Immunoblot Analysis
4.11. Molecular Docking
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Han, J.; Zhang, Y.; Pan, C.; Xian, Z.; Pan, C.; Zhao, Y.; Li, C.; Yi, Y.; Wang, L.; Tian, J.; et al. Forsythoside A and Forsythoside B Contribute to Shuanghuanglian Injection-Induced Pseudoallergic Reactions through the RhoA/ROCK Signaling Pathway. Int. J. Mol. Sci. 2019, 20, 6266. https://doi.org/10.3390/ijms20246266
Han J, Zhang Y, Pan C, Xian Z, Pan C, Zhao Y, Li C, Yi Y, Wang L, Tian J, et al. Forsythoside A and Forsythoside B Contribute to Shuanghuanglian Injection-Induced Pseudoallergic Reactions through the RhoA/ROCK Signaling Pathway. International Journal of Molecular Sciences. 2019; 20(24):6266. https://doi.org/10.3390/ijms20246266
Chicago/Turabian StyleHan, Jiayin, Yushi Zhang, Chen Pan, Zhong Xian, Chenling Pan, Yong Zhao, Chunying Li, Yan Yi, Lianmei Wang, Jingzhuo Tian, and et al. 2019. "Forsythoside A and Forsythoside B Contribute to Shuanghuanglian Injection-Induced Pseudoallergic Reactions through the RhoA/ROCK Signaling Pathway" International Journal of Molecular Sciences 20, no. 24: 6266. https://doi.org/10.3390/ijms20246266
APA StyleHan, J., Zhang, Y., Pan, C., Xian, Z., Pan, C., Zhao, Y., Li, C., Yi, Y., Wang, L., Tian, J., Liu, S., Wang, D., Meng, J., & Liang, A. (2019). Forsythoside A and Forsythoside B Contribute to Shuanghuanglian Injection-Induced Pseudoallergic Reactions through the RhoA/ROCK Signaling Pathway. International Journal of Molecular Sciences, 20(24), 6266. https://doi.org/10.3390/ijms20246266