Bioinformatics Analysis of the Lipoxygenase Gene Family in Radish (Raphanus sativus) and Functional Characterization in Response to Abiotic and Biotic Stresses
by
Jinglei Wang 1, Tianhua Hu 1, Wuhong Wang 1, Haijiao Hu 1, Qingzhen Wei 1, Xiaochun Wei 2 and Chonglai Bao 1,*
Jinglei Wang 1, Tianhua Hu 1, Wuhong Wang 1, Haijiao Hu 1, Qingzhen Wei 1, Xiaochun Wei 2 and Chonglai Bao 1,*
1
Institute of Vegetables Research, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2
Institute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(23), 6095; https://doi.org/10.3390/ijms20236095
Received: 27 September 2019 / Revised: 23 November 2019 / Accepted: 28 November 2019 / Published: 3 December 2019
(This article belongs to the Special Issue Mapping Abiotic Stress-Tolerance Genes in Plants)
Lipoxygenases (LOXs) are non-heme iron-containing dioxygenases involved in many developmental and stress-responsive processes in plants. However, little is known about the radish LOX gene family members and their functions in response to biotic and abiotic stresses. In this study, we completed a genome-wide analysis and expression profiling of RsLOX genes under abiotic and biotic stress conditions. We identified 11 RsLOX genes, which encoded conserved domains, and classified them in 9-LOX and 13-LOX categories according to their phylogenetic relationships. The characteristic structural features of 9-LOX and 13-LOX genes and the encoded protein domains as well as their evolution are presented herein. A qRT-PCR analysis of RsLOX expression levels in the roots under simulated drought, salinity, heat, and cold stresses, as well as in response to a Plasmodiophora brassicae infection, revealed three tandem-clustered RsLOX genes that are involved in responses to various environmental stresses via the jasmonic acid pathway. Our findings provide insights into the evolution and potential biological roles of RsLOXs related to the adaptation of radish to stress conditions.
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Keywords:
radish; lipoxygenase (LOX); gene family; abiotic stress; tandem duplication
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MDPI and ACS Style
Wang, J.; Hu, T.; Wang, W.; Hu, H.; Wei, Q.; Wei, X.; Bao, C. Bioinformatics Analysis of the Lipoxygenase Gene Family in Radish (Raphanus sativus) and Functional Characterization in Response to Abiotic and Biotic Stresses. Int. J. Mol. Sci. 2019, 20, 6095.
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