Next Article in Journal
Pairing and Exchanging between Daypyrum villosum Chromosomes 6V#2 and 6V#4 in the Hybrids of Two Different Wheat Alien Substitution Lines
Previous Article in Journal
Expression of Salivary and Serum Malondialdehyde and Lipid Profile of Patients with Periodontitis and Coronary Heart Disease
Previous Article in Special Issue
mTOR Links Tumor Immunity and Bone Metabolism: What are the Clinical Implications?
Open AccessArticle

Analysis of the Molecular Signaling Signatures of Muscle Protein Wasting Between the Intercostal Muscles and the Gastrocnemius Muscles in db/db Mice

by Kun Woo Kim 1,†, Mi-Ock Baek 2,3,4,†, Ji-Young Choi 3,†, Kuk Hui Son 1,* and Mee-Sup Yoon 2,3,4,*
1
Department of Thoracic and Cardiovascular Surgery, Gachon University Gil Medical Center, College of Medicine, Gachon University, Incheon 21565, Korea
2
Department of Molecular Medicine, School of Medicine, Gachon University, Incheon 21999, Korea
3
Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21936, Korea
4
Department of Health Sciences and Technology, GAIHST, Gachon University, Incheon 21999, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(23), 6062; https://doi.org/10.3390/ijms20236062
Received: 18 October 2019 / Revised: 22 November 2019 / Accepted: 28 November 2019 / Published: 1 December 2019
(This article belongs to the Special Issue mTOR in Human Diseases 2.0)
Type 2 diabetes (T2D) patients suffer from dyspnea, which contributes to disease-related morbidity. Although T2D has been reported to induce a catabolic state in skeletal muscle, whether T2D induces muscle wasting in respiratory muscles has not yet been investigated. In this study, we examine the difference in the molecular signaling signature of muscle wasting between the intercostal and gastrocnemius muscles using db/db mice, a well-known diabetic mouse model. Akt phosphorylation was significantly decreased in both the intercostal and gastrocnemius muscles of db/db mice and was accompanied by a decrease in mTORC1 activity. In addition, FoxO phosphorylation was suppressed, and ubiquitin-proteasome degradation, characterized by the level of Atrogin-1 and MuRF1, was subsequently enhanced in both muscle types of db/db mice. An increase in LC3BII levels and a decrease in p62 levels marked the occurrence of substantial autophagy in the gastrocnemius muscle but not in the intercostal muscles of db/db mice. Therefore, we suggest that the signaling events of muscle wasting in the intercostal muscles of db/db mice are different from those in the gastrocnemius muscle of db/db mice. View Full-Text
Keywords: muscle atrophy; intercostal muscles; gastrocnemius muscle; mTOR; Akt; FoxO; Atrogin-1; MuRF1; autophagic flux muscle atrophy; intercostal muscles; gastrocnemius muscle; mTOR; Akt; FoxO; Atrogin-1; MuRF1; autophagic flux
Show Figures

Figure 1

MDPI and ACS Style

Kim, K.W.; Baek, M.-O.; Choi, J.-Y.; Son, K.H.; Yoon, M.-S. Analysis of the Molecular Signaling Signatures of Muscle Protein Wasting Between the Intercostal Muscles and the Gastrocnemius Muscles in db/db Mice. Int. J. Mol. Sci. 2019, 20, 6062.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop