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Article

Wounding and UVB Light Synergistically Induce the Biosynthesis of Phenolic Compounds and Ascorbic Acid in Red Prickly Pears (Opuntia ficus-indica cv. Rojo Vigor)

1
Tecnologico de Monterrey, Escuela de Ingenieria y Ciencias, Av. General Ramon Corona 2514, Colonia Nuevo Mexico, Zapopan 45138, Jal., Mexico
2
Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843-2133, USA
3
Tecnologico de Monterrey, Escuela de Ingenieria y Ciencias, Av. Eugenio Garza Sada 2501 Sur, Colonia Tecnologico, Monterrey, NL 64849, Mexico
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(21), 5327; https://doi.org/10.3390/ijms20215327
Received: 14 October 2019 / Revised: 24 October 2019 / Accepted: 24 October 2019 / Published: 25 October 2019
(This article belongs to the Special Issue Bioactive Phenolics and Polyphenols 2018)
The present study evaluated the effects of ultraviolet B (UVB) radiation and wounding stress, applied alone or combined, on the biosynthesis of phenolic compounds and ascorbic acid in the peel and pulp of red prickly pear (Opuntia ficus-indica cv. Rojo Vigor). Whole and wounded-fruit samples were treated with UVB radiation (6.4 W·m−2) for 0 and 15 min, and stored for 24 h at 16 °C. Phytochemical analyses were performed separately in the peel and pulp. The highest phenolic accumulation occurred after storage of the whole tissue treated with UVB, where the main phenolic compounds accumulated in the peel and pulp were quercetin, sinapic acid, kaempferol, rosmarinic acid, and sinapoyl malate, showing increases of 709.8%, 570.2%, 442.8%, 439.9%, and 186.2%, respectively, as compared with the control before storage. Phenylalanine ammonia-lyase (PAL) activity was increased after storage of the whole and wounded tissue treated with UVB light, and this increase in PAL activity was associated to phenolic accumulation. On the other hand, l-galactono-γ-lactone dehydrogenase (GalLDH) activity and ascorbic acid biosynthesis was enhanced due to UVB radiation, and the effect was increased when UVB was applied in the wounded tissue showing 125.1% and 94.1% higher vitamin C content after storage when compared with the control. Respiration rate was increased due to wounding stress, whereas ethylene production was increased by wounding and UVB radiation in prickly pears. Results allowed the generation of a physiological model explaining the UVB and wound-induced accumulation of phenolic compounds and ascorbic acid in prickly pears, where wounding facilitates UVB to access the underlying tissue and enhances an apparent synergistic response. View Full-Text
Keywords: UVB light; wounding; phenolic compounds; ascorbic acid; l-galactono-γ-lactone dehydrogenase (GalLDH); phenylalanine ammonia-lyase (PAL); prickly pear UVB light; wounding; phenolic compounds; ascorbic acid; l-galactono-γ-lactone dehydrogenase (GalLDH); phenylalanine ammonia-lyase (PAL); prickly pear
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MDPI and ACS Style

Ortega-Hernández, E.; Nair, V.; Welti-Chanes, J.; Cisneros-Zevallos, L.; Jacobo-Velázquez, D.A. Wounding and UVB Light Synergistically Induce the Biosynthesis of Phenolic Compounds and Ascorbic Acid in Red Prickly Pears (Opuntia ficus-indica cv. Rojo Vigor). Int. J. Mol. Sci. 2019, 20, 5327. https://doi.org/10.3390/ijms20215327

AMA Style

Ortega-Hernández E, Nair V, Welti-Chanes J, Cisneros-Zevallos L, Jacobo-Velázquez DA. Wounding and UVB Light Synergistically Induce the Biosynthesis of Phenolic Compounds and Ascorbic Acid in Red Prickly Pears (Opuntia ficus-indica cv. Rojo Vigor). International Journal of Molecular Sciences. 2019; 20(21):5327. https://doi.org/10.3390/ijms20215327

Chicago/Turabian Style

Ortega-Hernández, Erika, Vimal Nair, Jorge Welti-Chanes, Luis Cisneros-Zevallos, and Daniel A. Jacobo-Velázquez 2019. "Wounding and UVB Light Synergistically Induce the Biosynthesis of Phenolic Compounds and Ascorbic Acid in Red Prickly Pears (Opuntia ficus-indica cv. Rojo Vigor)" International Journal of Molecular Sciences 20, no. 21: 5327. https://doi.org/10.3390/ijms20215327

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