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Open AccessArticle

The CCR4–NOT Deadenylase Complex Maintains Adipocyte Identity

1
Cell Signal Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa 904-0495, Japan
2
Nucleic Acid Chemistry and Engineering Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa 904-0495, Japan
3
Depatment of Biochemistry and Metabolic Science, Graduate School of Medicine, Akita University, Akita 010-8543, Japan
4
Laboratory for Immunogenetics, Center for Integrative Medical Sciences, RIKEN, Kanagawa 230-0045, Japan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(21), 5274; https://doi.org/10.3390/ijms20215274
Received: 27 September 2019 / Revised: 22 October 2019 / Accepted: 23 October 2019 / Published: 24 October 2019
(This article belongs to the Special Issue Adipose Stem Cells 2019)
Shortening of poly(A) tails triggers mRNA degradation; hence, mRNA deadenylation regulates many biological events. In the present study, we generated mice lacking the Cnot1 gene, which encodes an essential scaffold subunit of the CCR4–NOT deadenylase complex in adipose tissues (Cnot1-AKO mice) and we examined the role of CCR4–NOT in adipocyte function. Cnot1-AKO mice showed reduced masses of white adipose tissue (WAT) and brown adipose tissue (BAT), indicating abnormal organization and function of those tissues. Indeed, Cnot1-AKO mice showed hyperinsulinemia, hyperglycemia, insulin resistance, and glucose intolerance and they could not maintain a normal body temperature during cold exposure. Muscle-like fibrous material appeared in both WAT and BAT of Cnot1-AKO mice, suggesting the acquisition of non-adipose tissue characteristics. Gene expression analysis using RNA-sequencing (RNA-seq) showed that the levels of adipose tissue-related mRNAs, including those of metabolic genes, decreased, whereas the levels of inflammatory response-related mRNAs increased. These data suggest that the CCR4–NOT complex ensures proper adipose tissue function by maintaining adipocyte-specific mRNAs at appropriate levels and by simultaneously suppressing mRNAs that would impair adipocyte function if overexpressed. View Full-Text
Keywords: ccr4–not complex; adipocyte; lipodystrophy ccr4–not complex; adipocyte; lipodystrophy
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Takahashi, A.; Takaoka, S.; Kobori, S.; Yamaguchi, T.; Ferwati, S.; Kuba, K.; Yamamoto, T.; Suzuki, T. The CCR4–NOT Deadenylase Complex Maintains Adipocyte Identity. Int. J. Mol. Sci. 2019, 20, 5274.

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