Expression of Two α-Type Expansins from Ammopiptanthus nanus in Arabidopsis thaliana Enhance Tolerance to Cold and Drought Stresses
Abstract
:1. Introduction
2. Results
2.1. Cloning and Characterization of AnEXPA1 and AnEXPA2
2.2. AnEXPA1 and AnEXPA2 Showed Similar 3D Structure
2.3. Phylogenetic Analysis
2.4. Cis-Element Analysis of AnEXPA1 and AnEXPA2 Promoters
2.5. AnEXPA1 and AnEXPA2 Response to Different Abiotic Stresses and Hormone Induction
2.6. Promoters of AnEXPA1 and AnEXPA2 Showed Different Effects during Abiotic Stress and Hormone Induction
2.7. Overexpression of AnEXPA1 and AnEXPA2 in Arabidopsis Enhanced Tolerance to Cold Stress
2.8. Overexpression of AnEXPA1 and AnEXPA2 Enhanced Transgenic Plant Tolerance to Drought Stress
3. Discussion
3.1. AnEXPA1 and AnEXPA2 Responded to Different Abiotic Stresses and Hormone Signals
3.2. Overexpression of AnEXPA1 and AnEXPA2 in Arabidopsis Enhanced Tolerance to Cold and Drought Stresses
4. Materials and Methods
4.1. Plant Materials
4.2. RNA and DNA Extraction
4.3. Genome Walking
4.4. Generation of Plant Expression Vectors
4.5. GUS Dyeing and Protein Activity Assays
4.6. Arabidopsis Transgenic Plant Isolation
4.7. Cold and Drought Tolerance of Transgenic Plant Assays
4.8. Bioinformatics Analysis
4.9. qRT-PCR
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Liu, Y.; Zhang, L.; Hao, W.; Zhang, L.; Liu, Y.; Chen, L. Expression of Two α-Type Expansins from Ammopiptanthus nanus in Arabidopsis thaliana Enhance Tolerance to Cold and Drought Stresses. Int. J. Mol. Sci. 2019, 20, 5255. https://doi.org/10.3390/ijms20215255
Liu Y, Zhang L, Hao W, Zhang L, Liu Y, Chen L. Expression of Two α-Type Expansins from Ammopiptanthus nanus in Arabidopsis thaliana Enhance Tolerance to Cold and Drought Stresses. International Journal of Molecular Sciences. 2019; 20(21):5255. https://doi.org/10.3390/ijms20215255
Chicago/Turabian StyleLiu, Yanping, Li Zhang, Wenfang Hao, Ling Zhang, Yi Liu, and Longqing Chen. 2019. "Expression of Two α-Type Expansins from Ammopiptanthus nanus in Arabidopsis thaliana Enhance Tolerance to Cold and Drought Stresses" International Journal of Molecular Sciences 20, no. 21: 5255. https://doi.org/10.3390/ijms20215255