Highly Selective Cleavage of TH2-Promoting Cytokines by the Human and the Mouse Mast Cell Tryptases, Indicating a Potent Negative Feedback Loop on TH2 Immunity
Abstract
:1. Introduction
2. Results
2.1. Analysis of the Purity and Activity of the Recombinant Human and Mouse Mast Cell Tryptases
2.2. Analysis of Cleavage Sensitivity against a Panel of 69 Cytokines and Chemokines by the Recombinant Human Tryptase
2.3. Analysis of Cleavage Sensitivity against a Panel of 56 Cytokines and Chemokines by the Recombinant Mouse Tryptase, mMCP-6
2.4. Analysis of Structural Similarities between the Three Efficiently Cleaved Cytokines and Chemokines
2.5. Analysis of the Effect of Spermine on Tryptase Activity
2.6. Cleavage of other Substrates by Human Tryptase
2.7. Cleavage Selectivity of Wild Type (WT) and Mutant Human Tryptase
3. Discussion
4. Materials and Methods
4.1. Enzymes and other Reagents
Recombinant Human and Mouse Cytokines and Chemokines
4.2. Analysis of the Sensitivity to Cleavage by the Recombinant Human and Mouse Tryptase
4.3. Analysis of the Cleavage of Human Fibrinogen and Fibronectin
Author Contributions
Funding
Conflicts of Interest
References
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Cytokine | hTryp. | M6 | HC | Cytokine | hTryp. | M6 | HC |
---|---|---|---|---|---|---|---|
IL-4 | − | − | − | IL-1α | − | − | |
IL-5 | − | − | − | IL-1β | − | − | − |
IL-10 | − | − | − | IL-1RA | + | ||
TSLP | ++++ | − | + | IL-33 | − | + | ++++ |
IL-13 | − | +++ | + | IL-18 | − | +++ | |
SCF | − | − | − | IGF-1 | − | ++++ | |
IL-3 | − | − | + | IGF-2 | − | ||
IL-31 | − | − | − | HGF | − | ||
GM-CSF | − | + | − | IL-8 | ++ | − | |
M-CSF | − | − | IP-10 | − | ++++ | − | |
G-CSF | − | − | − | MCP-1 | − | + | − |
TGF- β3 | − | MCP-2 | − | − | |||
TNF-α | − | − | − | MCP-3 | ++++ | − | |
IL-6 | − | + | ++ | RANTES | − | − | − |
IL-11 | − | + | + | SDF-1α | − | ++ | − |
LIF | − | − | + | SDF-1β | + | − | − |
IL-2 | − | − | − | EGF | − | − | − |
IFN-γ | +++ | + | + | MIP-1α | − | − | - |
IL-15 | − | − | ++++ | flt3L | − | + | ++ |
IL-12 | − | − | FGF-9 | − | |||
IL-20 | + | − | − | FGF-19 | − | ||
IL-7 | + | + | − | BMP-14 | − | ||
IL-21 | ++++ | ++++ | − | GAL-7H | − | ||
IL-16 | − | − | pF4V1 | − | |||
VEGF-A | + | ++++ | − | IL-25 | − | − | − |
VEGF-121 | − | IL-9 | − | ++++ | |||
PDGF-A | − | + | − | MIP-3a | − | ++++ | |
PDGF-B | − | +++ | − | MIP-3b | ++++ | ++++ | |
BMP-2 | − | Eotaxin | +++ | ++++ | |||
CTGF | ++ | + | IL-27A | - | |||
FGF-1 | + | − | + | IL17C | ++++ | ||
FGF-2 | − | + | + | NGF-β | − | ||
IL-17A | + | − | − | TPO | + | ||
IL-17F | + | + | − | GRO-a | − | ||
IL-22 | − | − | − | GRO-b | − | ||
CD40L | − | + | − | MIP-1b | − | ||
BAFF | − | + | |||||
IL-19 | − | − | − | ||||
sIL-6R | − | ||||||
CNTF | − |
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Fu, Z.; Akula, S.; Thorpe, M.; Hellman, L. Highly Selective Cleavage of TH2-Promoting Cytokines by the Human and the Mouse Mast Cell Tryptases, Indicating a Potent Negative Feedback Loop on TH2 Immunity. Int. J. Mol. Sci. 2019, 20, 5147. https://doi.org/10.3390/ijms20205147
Fu Z, Akula S, Thorpe M, Hellman L. Highly Selective Cleavage of TH2-Promoting Cytokines by the Human and the Mouse Mast Cell Tryptases, Indicating a Potent Negative Feedback Loop on TH2 Immunity. International Journal of Molecular Sciences. 2019; 20(20):5147. https://doi.org/10.3390/ijms20205147
Chicago/Turabian StyleFu, Zhirong, Srinivas Akula, Michael Thorpe, and Lars Hellman. 2019. "Highly Selective Cleavage of TH2-Promoting Cytokines by the Human and the Mouse Mast Cell Tryptases, Indicating a Potent Negative Feedback Loop on TH2 Immunity" International Journal of Molecular Sciences 20, no. 20: 5147. https://doi.org/10.3390/ijms20205147