Identification of an Interferon-Stimulated Long Noncoding RNA (LncRNA ISR) Involved in Regulation of Influenza A Virus Replication

Pan, Qidong; Zhao, Zhonghui; Liao, Yuan; Chiu, Shih-Hsin; Wang, Song; Chen, Biao; Chen, Na; Chen, Yuhai; Chen, Ji-Long

doi:10.3390/ijms20205118

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Identification of an Interferon-Stimulated Long Noncoding RNA (LncRNA ISR) Involved in Regulation of Influenza A Virus Replication

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Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China

CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

Author to whom correspondence should be addressed.

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These authors contributed equally to this work.

Int. J. Mol. Sci. 2019, 20(20), 5118; https://doi.org/10.3390/ijms20205118

Received: 2 July 2019 / Revised: 3 October 2019 / Accepted: 6 October 2019 / Published: 16 October 2019

(This article belongs to the Special Issue Computational Models in Non-Coding RNA and Human Disease)

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Abstract

Long noncoding RNAs (lncRNAs) are involved in a diversity of biological processes. It is known that differential expression of thousands of lncRNAs occurs in host during influenza A virus (IAV) infection. However, only few of them have been well characterized. Here, we identified a lncRNA, named as interferon (IFN)-stimulated lncRNA (ISR), which can be significantly upregulated in response to IAV infection in a mouse model. A sequence alignment revealed that lncRNA ISR is present in mice and human beings, and indeed, we found that it was expressed in several human and mouse cell lines and tissues. Silencing lncRNA ISR in A549 cells resulted in a significant increase in IAV replication, whereas ectopic expression of lncRNA ISR reduced the viral replication. Interestingly, interferon-β (IFN-β) treatment was able to induce lncRNA ISR expression, and induction of lncRNA ISR by viral infection was nearly abolished in host deficient of IFNAR1, a type I IFN receptor. Furthermore, the level of IAV-induced lncRNA ISR expression was decreased either in retinoic acid-inducible gene I (RIG-I) knockout A549 cells and mice or by nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) inhibitor treatment. Together, these data elucidate that lncRNA ISR is regulated by RIG-I-dependent signaling that governs IFN-β production during IAV infection, and has an inhibitory capacity in viral replication. View Full-Text

Keywords: Influenza A virus; long noncoding RNAs; viral replication; interferon; innate immunity

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Pan, Q.; Zhao, Z.; Liao, Y.; Chiu, S.-H.; Wang, S.; Chen, B.; Chen, N.; Chen, Y.; Chen, J.-L. Identification of an Interferon-Stimulated Long Noncoding RNA (LncRNA ISR) Involved in Regulation of Influenza A Virus Replication. Int. J. Mol. Sci. 2019, 20, 5118. https://doi.org/10.3390/ijms20205118

AMA Style

Pan Q, Zhao Z, Liao Y, Chiu S-H, Wang S, Chen B, Chen N, Chen Y, Chen J-L. Identification of an Interferon-Stimulated Long Noncoding RNA (LncRNA ISR) Involved in Regulation of Influenza A Virus Replication. International Journal of Molecular Sciences. 2019; 20(20):5118. https://doi.org/10.3390/ijms20205118

Chicago/Turabian Style

Pan, Qidong, Zhonghui Zhao, Yuan Liao, Shih-Hsin Chiu, Song Wang, Biao Chen, Na Chen, Yuhai Chen, and Ji-Long Chen. 2019. "Identification of an Interferon-Stimulated Long Noncoding RNA (LncRNA ISR) Involved in Regulation of Influenza A Virus Replication" International Journal of Molecular Sciences 20, no. 20: 5118. https://doi.org/10.3390/ijms20205118

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Identification of an Interferon-Stimulated Long Noncoding RNA (LncRNA ISR) Involved in Regulation of Influenza A Virus Replication

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