ATP-Evoked Intracellular Ca2+ Responses in M-CSF Differentiated Human Monocyte-Derived Macrophage are Mediated by P2X4 and P2Y11 Receptor Activation
Abstract
:1. Introduction
2. Results
2.1. ATP Evokes Intracellular Ca2+ Responses in M-CSF Differentiated Macrophage
2.2. M-CSF Differentiated Macrophage Express Multiple P2Y and P2X Receptor Subtypes
2.3. P2Y11 and P2X4 Mediate ATP-Evoked Ca2+ Responses
3. Discussion
4. Materials and Methods
4.1. Peripheral Blood Mononuclear Cells (PBMC) Isolation
4.2. Generation of Monocyte-Derived Macrophage
4.3. Quantitative RT-PCR
4.4. Immunocytochemistry
4.5. Flow Cytometry
4.6. Intracellular Ca2+ Measurement
4.7. Drugs and Application
4.8. Data and Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Layhadi, J.A.; Fountain, S.J. ATP-Evoked Intracellular Ca2+ Responses in M-CSF Differentiated Human Monocyte-Derived Macrophage are Mediated by P2X4 and P2Y11 Receptor Activation. Int. J. Mol. Sci. 2019, 20, 5113. https://doi.org/10.3390/ijms20205113
Layhadi JA, Fountain SJ. ATP-Evoked Intracellular Ca2+ Responses in M-CSF Differentiated Human Monocyte-Derived Macrophage are Mediated by P2X4 and P2Y11 Receptor Activation. International Journal of Molecular Sciences. 2019; 20(20):5113. https://doi.org/10.3390/ijms20205113
Chicago/Turabian StyleLayhadi, Janice A., and Samuel J. Fountain. 2019. "ATP-Evoked Intracellular Ca2+ Responses in M-CSF Differentiated Human Monocyte-Derived Macrophage are Mediated by P2X4 and P2Y11 Receptor Activation" International Journal of Molecular Sciences 20, no. 20: 5113. https://doi.org/10.3390/ijms20205113