Glycyrrhizin Acid and Glycyrrhetinic Acid Modified Polyethyleneimine for Targeted DNA Delivery to Hepatocellular Carcinoma
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and Characterization of GA or GL Functionalized PEIs
2.2. Biophysical Characterization of GA or GL Modified PEIs
2.3. Cytotoxicity and Gene Transfection in Vitro
2.4. Effect of free GA on the Transfection Efficiency
2.5. Endocytic Pathway and Intracellular Trafficking
2.6. In Vivo Gene Transfection
3. Materials and Methods
3.1. Materials and Cell Culture
3.2. Preparation and Characterization of PEI-GA and PEI-GL Polyplexes
3.3. Gel Retardation Assay
3.4. Cytotoxicity of PEI-GA and PEI-GL Conjugates
3.5. Evaluation of Transfection Efficiency in Vitro
3.6. Free GA Competition Assay
3.7. Endocytosis Inhibitor Effects on Cellular Uptake and Transfection
3.8. Observation of Intracellular Trafficking by Confocal Imaging
3.9. Transfection in Vivo
3.10. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Item | Feed Ratio | Substitution Amount | Yield | |
---|---|---|---|---|
(by OD) | (by 1HNMR) | |||
PEI:GA | 1:1 | 0.75 | 0.69 | 67.8% |
1:2 | 1.10 | 1.16 | 54.1% | |
1:3 | 1.71 | 1.89 | 45.8% | |
PEI:GL1 | 1:1 | 0.62 | 0.66 | 51.6% |
PEI:GL2 | 1:1 | 0.65 | 0.67 | 52.8% |
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Cao, M.; Gao, Y.; Zhan, M.; Qiu, N.; Piao, Y.; Zhou, Z.; Shen, Y. Glycyrrhizin Acid and Glycyrrhetinic Acid Modified Polyethyleneimine for Targeted DNA Delivery to Hepatocellular Carcinoma. Int. J. Mol. Sci. 2019, 20, 5074. https://doi.org/10.3390/ijms20205074
Cao M, Gao Y, Zhan M, Qiu N, Piao Y, Zhou Z, Shen Y. Glycyrrhizin Acid and Glycyrrhetinic Acid Modified Polyethyleneimine for Targeted DNA Delivery to Hepatocellular Carcinoma. International Journal of Molecular Sciences. 2019; 20(20):5074. https://doi.org/10.3390/ijms20205074
Chicago/Turabian StyleCao, Mingzhuo, Yong Gao, Mengling Zhan, Nasha Qiu, Ying Piao, Zhuxian Zhou, and Youqing Shen. 2019. "Glycyrrhizin Acid and Glycyrrhetinic Acid Modified Polyethyleneimine for Targeted DNA Delivery to Hepatocellular Carcinoma" International Journal of Molecular Sciences 20, no. 20: 5074. https://doi.org/10.3390/ijms20205074