Renal Artery Stenosis Alters Gene Expression in Swine Scattered Tubular-Like Cells
Abstract
:1. Introduction
2. Results
2.1. Systemic Characteristics and Renal Function
2.2. STC Characterization
2.3. mRNAseq Analysis
2.4. Genes Upregulated in RAS-STCs
2.5. Genes Downregulated in RAS-STCs
2.6. Validation of mRNAseq Analysis
2.7. STC Activation and Proliferation
3. Discussion
4. Materials and Methods
4.1. Experimental Design
4.2. STC Isolation and Characterization
4.3. mRNAseq and Bioinformatic Analysis
4.4. Validation of mRNAseq Analysis
4.5. STCs Activation
4.6. STC Proliferation
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviation
AKI | Acute kidney injury |
STCs | Scattered tubular-like cells |
RAS | Renal artery stenosis |
RBF | Renal blood flow |
mRNAseq | Messenger RNA sequencing |
MDCT | Multi-detector computed tomography |
GFR | Glomerular filtration rate |
CPM | Counts per million |
qPCR | Quantitative-polymerase chain reaction |
BCAM | Linear dichroism basal cell adhesion molecule |
CADH3 | Cadherin 3 |
CADH16 | Cadherin 6 |
EpCAM | Epithelial cellular adhesion molecule |
COL6A2 | Collagen 6A2 |
COL18A1 | Collagen 18A1 |
LAMA5 | Laminin A5 |
LAMB3 | Laminin B3 |
POU3F3 | POU Class 3 Homeobox 3 |
SOX | Sex determining region Y-box |
PANTHER | Protein Analysis Through Evolutionary Relationships |
DAVID | Database for Annotation, Visualization, and Integrated Discovery |
STRING | Search Tool for the Retrieval of Interacting Genes |
PHA-E | Phaseolus vulgaris erythroagglutinin |
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Parameter | Normal | RAS |
---|---|---|
Body Weight (Kg) | 50.3 ± 0.6 | 54.3 ± 5.5 |
Degree of stenosis (%) | 0 | 84.7 ± 12.7 * |
Systolic blood pressure (mmHg) | 96.3 ± 5.1 | 151.7 ± 33.3 * |
Diastolic blood pressure (mmHg) | 70.0 ± 6.0 | 118.7 ± 21.7 * |
Mean arterial pressure (mmHg) | 78.8 ± 5.3 | 129.7 ± 25.5 * |
Serum creatinine (mg/dL) | 1.2 ± 0.1 | 1.8 ± 0.3 * |
Cortical volume (mL) | 104.8 ± 4.4 | 58.7 ± 27.1 * |
Medullary volume (mL) | 19.6 ± 1.0 | 19.5 ± 2.3 |
Cortical perfusion (mL/min/mL tissue) | 5.4 ± 0.4 | 2.4 ± 0.5 * |
Medullary perfusion (mL/min/mL tissue) | 2.9 ± 0.3 | 2.7 ± 0.3 |
RBF (mL/min) | 607.4 ± 42.4 | 315.5 ± 94.4 * |
GFR (mL/min) | 98.1 ± 9.5 | 52.2 ± 8.3 * |
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Aghajani Nargesi, A.; Zhu, X.-Y.; Liu, Y.; Tang, H.; Jordan, K.L.; Lerman, L.O.; Eirin, A. Renal Artery Stenosis Alters Gene Expression in Swine Scattered Tubular-Like Cells. Int. J. Mol. Sci. 2019, 20, 5069. https://doi.org/10.3390/ijms20205069
Aghajani Nargesi A, Zhu X-Y, Liu Y, Tang H, Jordan KL, Lerman LO, Eirin A. Renal Artery Stenosis Alters Gene Expression in Swine Scattered Tubular-Like Cells. International Journal of Molecular Sciences. 2019; 20(20):5069. https://doi.org/10.3390/ijms20205069
Chicago/Turabian StyleAghajani Nargesi, Arash, Xiang-Yang Zhu, Yuanhang Liu, Hui Tang, Kyra L. Jordan, Lilach O. Lerman, and Alfonso Eirin. 2019. "Renal Artery Stenosis Alters Gene Expression in Swine Scattered Tubular-Like Cells" International Journal of Molecular Sciences 20, no. 20: 5069. https://doi.org/10.3390/ijms20205069