Angiotensin II Influences Pre-mRNA Splicing Regulation by Enhancing RBM20 Transcription Through Activation of the MAPK/ELK1 Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. Splicing Regulation of RBM20 in Z-, I-, and M-Band Titin at Different Ages
2.2. Impact of Hormones on the Regulation of Z-, I-, and M-Band Titin Splicing with and without RBM20
2.3. Hormone Impact on the Splicing of Other Substrates
2.4. Activation of MAPK Signaling by Insulin, Thyroid Hormone, and Ang II in Cultured Neonatal Cardiomyocytes
2.5. MAPK Signaling Activation Enhances RBM20 Transcription in Primary Neonatal Cardiomyocyte Cultures
3. Discussion
4. Materials and Methods
4.1. Experimental Animals and Tissue Samples
4.2. Neonatal Rat Cardiomyocytes (NRCMs) Isolation and Treatment
4.3. Reverse Transcriptional PCR (RT-PCR) and Quantitative Real-Time PCR (qPCR) Analysis
4.4. Western Blot Analysis
4.5. Bioinformatics Analysis
4.6. Dual-Luciferase Activity Assay
4.7. Statistics
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
T3 | Triiodothyronine |
Ang II | Angiotensin II |
WT | Wild type |
KO | RBM20 knockout |
STZ | Streptozotocin |
RBM20 | RNA binding motif 20 |
SR | Serine-arginine |
PTU | Propylthiouracil |
Camk2g | Calcium/calmodulin dependent protein kinase II gamma |
Ldb3 | LIM domain binding 3 |
Trdn | Triadin |
MAPK | Mitogen-Activated Protein Kinase |
SD | Sprague Dawley |
BN | Brown Norway |
GAPDH | Glyceraldehyde-3-phosphate |
SE | Standard error |
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Cai, H.; Zhu, C.; Chen, Z.; Maimaiti, R.; Sun, M.; McCormick, R.J.; Lan, X.; Chen, H.; Guo, W. Angiotensin II Influences Pre-mRNA Splicing Regulation by Enhancing RBM20 Transcription Through Activation of the MAPK/ELK1 Signaling Pathway. Int. J. Mol. Sci. 2019, 20, 5059. https://doi.org/10.3390/ijms20205059
Cai H, Zhu C, Chen Z, Maimaiti R, Sun M, McCormick RJ, Lan X, Chen H, Guo W. Angiotensin II Influences Pre-mRNA Splicing Regulation by Enhancing RBM20 Transcription Through Activation of the MAPK/ELK1 Signaling Pathway. International Journal of Molecular Sciences. 2019; 20(20):5059. https://doi.org/10.3390/ijms20205059
Chicago/Turabian StyleCai, Hanfang, Chaoqun Zhu, Zhilong Chen, Rexiati Maimaiti, Mingming Sun, Richard J. McCormick, Xianyong Lan, Hong Chen, and Wei Guo. 2019. "Angiotensin II Influences Pre-mRNA Splicing Regulation by Enhancing RBM20 Transcription Through Activation of the MAPK/ELK1 Signaling Pathway" International Journal of Molecular Sciences 20, no. 20: 5059. https://doi.org/10.3390/ijms20205059