Abscisic Acid Represses Rice Lamina Joint Inclination by Antagonizing Brassinosteroid Biosynthesis and Signaling
Abstract
:1. Introduction
2. Results
2.1. ABA Antagonizes the Promotion of Lamina Joint Inclination by BR in Rice
2.2. Modulating the Expression of BR Biosynthesis Genes Plays an Important Role in ABA Regulation of Lamina Joint Inclination
2.3. Interaction between ABA and BR Biosynthesis Pathways in Lamina Joint Inclination
2.4. Identification of the Genes Commonly Responsive to BR and ABA via RNA-Seq
2.5. Suppression of Lamina Joint Inclination by ABA Does Not Depend on BR Perception
2.6. BR and ABA Crosstalk in Establishing Lamina Joint Angle Is Partially Mediated by GSK2
2.7. DLT Represents a Major ABA–BR Crosstalk Node in Coordinating Lamina Joint Inclination
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Lamina Joint Inclination Assay
4.3. Quantitative Real-Time PCR (qRT-PCR) Analysis
4.4. RNA-Seq Analysis
4.5. Bioinformatic Analysisand qRT-PCR Validation of RNA-Seq Data
4.6. Yeast Two-Hybrid Assays
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ABA | abscisic acid |
ABIL1 | ABI-like 1 |
ANOVA | analysis of variance |
BIN2 | BR-insensitive 2 |
BL | brassinolide |
BR | brassinosteroid |
BU1 | brassinosteroid upregulated1 |
BZR1 | brassinazole resistant1 |
D2 | ebisud warf |
D11 | dwarf 11 |
DEGs | differentially expressed genes |
DLT | dwarf and low-tillering |
DMSO | dimethylsulfoxide |
GO | gene ontology |
GSK2 | glycogen synthase kinase 2 |
LAI | leaf area index |
OsBRI1 | brassinosteroid-insensitive1 |
OsDWF4 | osdwarf 4 |
OsXTH1 | xyloglucan endotransglycosylase/hydrolase 1 |
PHI-1 | phosphate-induced protein-1 |
qRT-PCR | quantitative real-time polymerase chain reaction |
QTLs | quantitative trait loci |
RNA-Seq | RNA sequencing |
SD | standard deviation |
SPL | Squamosa-promoter binding protein-like |
UBC | ubiquitin-conjugating enzyme |
UPA1 | upright plant architecture1 |
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Li, Q.-F.; Lu, J.; Zhou, Y.; Wu, F.; Tong, H.-N.; Wang, J.-D.; Yu, J.-W.; Zhang, C.-Q.; Fan, X.-L.; Liu, Q.-Q. Abscisic Acid Represses Rice Lamina Joint Inclination by Antagonizing Brassinosteroid Biosynthesis and Signaling. Int. J. Mol. Sci. 2019, 20, 4908. https://doi.org/10.3390/ijms20194908
Li Q-F, Lu J, Zhou Y, Wu F, Tong H-N, Wang J-D, Yu J-W, Zhang C-Q, Fan X-L, Liu Q-Q. Abscisic Acid Represses Rice Lamina Joint Inclination by Antagonizing Brassinosteroid Biosynthesis and Signaling. International Journal of Molecular Sciences. 2019; 20(19):4908. https://doi.org/10.3390/ijms20194908
Chicago/Turabian StyleLi, Qian-Feng, Jun Lu, Yu Zhou, Fan Wu, Hong-Ning Tong, Jin-Dong Wang, Jia-Wen Yu, Chang-Quan Zhang, Xiao-Lei Fan, and Qiao-Quan Liu. 2019. "Abscisic Acid Represses Rice Lamina Joint Inclination by Antagonizing Brassinosteroid Biosynthesis and Signaling" International Journal of Molecular Sciences 20, no. 19: 4908. https://doi.org/10.3390/ijms20194908