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Article

Abscisic Acid Represses Rice Lamina Joint Inclination by Antagonizing Brassinosteroid Biosynthesis and Signaling

1
Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, College of Agriculture, Yangzhou University, Yangzhou 225009, China
2
Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province/Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Yangzhou University, Yangzhou 225009, China
3
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(19), 4908; https://doi.org/10.3390/ijms20194908
Received: 7 September 2019 / Revised: 26 September 2019 / Accepted: 1 October 2019 / Published: 3 October 2019
(This article belongs to the Section Molecular Plant Sciences)
Leaf angle is a key parameter that determines plant architecture and crop yield. Hormonal crosstalk involving brassinosteroid (BR) plays an essential role in leaf angle regulation in cereals. In this study, we investigated whether abscisic acid (ABA), an important stress-responsive hormone, co-regulates lamina joint inclination together with BR, and, if so, what the underlying mechanism is. Therefore, lamina joint inclination assay and RNA sequencing (RNA-Seq) analysis were performed here. ABA antagonizes the promotive effect of BR on leaf angle. Hundreds of genes responsive to both hormones that are involved in leaf-angle determination were identified by RNA-Seq and the expression of a gene subset was confirmed using quantitative real-time PCR (qRT-PCR). Results from analysis of rice mutants or transgenic lines affected in BR biosynthesis and signaling indicated that ABA antagonizes the effect of BR on lamina joint inclination by targeting the BR biosynthesis gene D11 and BR signaling genes GSK2 and DLT, thus forming a multi-level regulatory module that controls leaf angle in rice. Taken together, our findings demonstrate that BR and ABA antagonistically regulate lamina joint inclination in rice, thus contributing to the elucidation of the complex hormonal interaction network that optimizes leaf angle in rice. View Full-Text
Keywords: abcisic acid; brassinosteroid; hormonal crosstalk; lamina joint inclination; Oryza sativa L.; RNA-Seq abcisic acid; brassinosteroid; hormonal crosstalk; lamina joint inclination; Oryza sativa L.; RNA-Seq
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MDPI and ACS Style

Li, Q.-F.; Lu, J.; Zhou, Y.; Wu, F.; Tong, H.-N.; Wang, J.-D.; Yu, J.-W.; Zhang, C.-Q.; Fan, X.-L.; Liu, Q.-Q. Abscisic Acid Represses Rice Lamina Joint Inclination by Antagonizing Brassinosteroid Biosynthesis and Signaling. Int. J. Mol. Sci. 2019, 20, 4908. https://doi.org/10.3390/ijms20194908

AMA Style

Li Q-F, Lu J, Zhou Y, Wu F, Tong H-N, Wang J-D, Yu J-W, Zhang C-Q, Fan X-L, Liu Q-Q. Abscisic Acid Represses Rice Lamina Joint Inclination by Antagonizing Brassinosteroid Biosynthesis and Signaling. International Journal of Molecular Sciences. 2019; 20(19):4908. https://doi.org/10.3390/ijms20194908

Chicago/Turabian Style

Li, Qian-Feng, Jun Lu, Yu Zhou, Fan Wu, Hong-Ning Tong, Jin-Dong Wang, Jia-Wen Yu, Chang-Quan Zhang, Xiao-Lei Fan, and Qiao-Quan Liu. 2019. "Abscisic Acid Represses Rice Lamina Joint Inclination by Antagonizing Brassinosteroid Biosynthesis and Signaling" International Journal of Molecular Sciences 20, no. 19: 4908. https://doi.org/10.3390/ijms20194908

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