Differences in Expression of Genes Involved in Bone Development and Morphogenesis in the Walls of Internal Thoracic Artery and Saphenous Vein Conduits May Provide Markers Useful for Evaluation Graft Patency
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Human Subjects
4.2. Operation Procedure and Sample Collection
4.3. RNA Extraction and Reverse Transcription
4.4. Microarray Expression Study and Data Analysis
4.5. Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) Analysis
4.6. Histological Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Gene Symbol | Ratio | Adjusted p Value | Entrez Gene ID |
---|---|---|---|
SHOX2 | −22.8240429 | 1.03 × 10−27 | 6474 |
HOXA11 | −9.830934198 | 7.60 × 10−17 | 3207 |
COMP | −7.773021124 | 3.33 × 10−13 | 1311 |
TNC | −7.430294969 | 1.88 × 10−08 | 3371 |
GDF10 | −5.309648509 | 2.16 × 10−08 | 2662 |
MSX1 | −3.455950944 | 9.17 × 10−13 | 4487 |
CTHRC1 | −3.018066571 | 5.20 × 10−11 | 115908 |
CSGALNACT1 | −2.881617301 | 2.10 × 10−12 | 55790 |
HOXA10 | −2.63199568 | 1.36 × 10−12 | 3206 |
KL | −2.458105612 | 2.59 × 10−08 | 9365 |
HOXC10 | −2.457042843 | 7.09 × 10−15 | 3226 |
BMP6 | −2.45216404 | 2.98 × 10−07 | 654 |
IFITM1 | −2.349841846 | 2.37 × 10−08 | 8519 |
RUNX2 | −2.331169268 | 5.34 × 10−07 | 860 |
PTGER4 | −2.280369849 | 7.12 × 10−09 | 5734 |
FHL2 | −2.216349206 | 6.35 × 10−10 | 2274 |
HOXA13 | −2.166453813 | 2.16 × 10−10 | 3209 |
TP53INP2 | −2.127154082 | 3.09 × 10−08 | 58476 |
CD44 | −1.387817152 | 0.006540227 | 960 |
FLI1 | –1.131519079 | 0.002636506 | 2313 |
ROR2 | −1.128865754 | 0.004346143 | 4920 |
COL11A1 | −1.12582207 | 0.000190115 | 1301 |
ADAMTS12 | −1.054115576 | 0.188720272 | 81792 |
GPR68 | −1.028071249 | 0.512393039 | 8111 |
CSRNP1 | 1.338139052 | 0.026644084 | 64651 |
BCAP29 | 1.823380549 | 3.52 × 10−07 | 55973 |
TOB1 | 1.841885719 | 1.99 × 10−08 | 10140 |
STC1 | 1.845964521 | 0.000109973 | 6781 |
HOXA2 | 2.01745347 | 5.20 × 10−11 | 3199 |
HOXB7 | 2.230080809 | 3.31 × 10−09 | 3217 |
HOXB2 | 2.433099517 | 5.86 × 10−11 | 3212 |
LRRC17 | 2.543411445 | 2.26 × 10−10 | 10234 |
SPP1 | 2.96640349 | 0.001152397 | 6696 |
TAC1 | 3.167584851 | 4.92 × 10−05 | 6863 |
RBP4 | 3.228449588 | 3.61 × 10−05 | 5950 |
ENPP1 | 3.320786599 | 4.83 × 10−10 | 5167 |
TNFRSF11B | 4.741562441 | 1.54 × 10−10 | 4982 |
NPR3 | 5.295452922 | 2.59 × 10−12 | 4883 |
COL13A1 | 8.475105942 | 8.83 × 10−18 | 1305 |
SOST | 12.36428646 | 1.01 × 10−10 | 50964 |
Gene | Primer Sequence (5′–3′) | Product Size (bp) | |
---|---|---|---|
SHOX2 | F | GAAGGCCAGACCAAAATCAA | 234 |
R | GGCCCCTATGAGAACACCTT | ||
RUNX2 | F | GGACGAGGCAAGAGTTTCAC | 165 |
R | GAGGCGGTCAGAGAACAAAC | ||
BMP6 | F | AAGAAGGCTGGCTGGAATTT | 170 |
R | GAAGGGCTGCTTGTCGTAAG | ||
STC1 | F | TGATCAGTGCTTCTGCAACC | 242 |
R | GACGAATGCTTTTCCCTGAG | ||
CSGALNACT1 | F | CAGAAAGGGACAAAGGGACA | 243 |
R | TGAGATGGACTCTCCCATCC | ||
COL13A1 | F | CAAAGGGAGAAGCAGGTGTC | 175 |
R | TCCTGGAGAGCCTCATTGAT |
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Nawrocki, M.J.; Perek, B.; Sujka-Kordowska, P.; Konwerska, A.; Kałużna, S.; Zawierucha, P.; Bruska, M.; Zabel, M.; Jemielity, M.; Nowicki, M.; et al. Differences in Expression of Genes Involved in Bone Development and Morphogenesis in the Walls of Internal Thoracic Artery and Saphenous Vein Conduits May Provide Markers Useful for Evaluation Graft Patency. Int. J. Mol. Sci. 2019, 20, 4890. https://doi.org/10.3390/ijms20194890
Nawrocki MJ, Perek B, Sujka-Kordowska P, Konwerska A, Kałużna S, Zawierucha P, Bruska M, Zabel M, Jemielity M, Nowicki M, et al. Differences in Expression of Genes Involved in Bone Development and Morphogenesis in the Walls of Internal Thoracic Artery and Saphenous Vein Conduits May Provide Markers Useful for Evaluation Graft Patency. International Journal of Molecular Sciences. 2019; 20(19):4890. https://doi.org/10.3390/ijms20194890
Chicago/Turabian StyleNawrocki, Mariusz J., Bartłomiej Perek, Patrycja Sujka-Kordowska, Aneta Konwerska, Sandra Kałużna, Piotr Zawierucha, Małgorzata Bruska, Maciej Zabel, Marek Jemielity, Michał Nowicki, and et al. 2019. "Differences in Expression of Genes Involved in Bone Development and Morphogenesis in the Walls of Internal Thoracic Artery and Saphenous Vein Conduits May Provide Markers Useful for Evaluation Graft Patency" International Journal of Molecular Sciences 20, no. 19: 4890. https://doi.org/10.3390/ijms20194890