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Open AccessArticle

CircRNA Expression Pattern and ceRNA and miRNA–mRNA Networks Involved in Anther Development in the CMS Line of Brassica campestris

by Yuwei Liang 1,2, Yuzhi Zhang 1,2, Liai Xu 1,2, Dong Zhou 1,2, Zongmin Jin 1,2, Huiyan Zhou 1,2, Sue Lin 3, Jiashu Cao 1,2 and Li Huang 1,2,*
1
Laboratory of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, China
2
Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture/Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Hangzhou 310058, China
3
Institute of Life Sciences, Wenzhou University, Wenzhou 325000, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(19), 4808; https://doi.org/10.3390/ijms20194808
Received: 3 September 2019 / Revised: 20 September 2019 / Accepted: 24 September 2019 / Published: 27 September 2019
(This article belongs to the Collection Genetics and Molecular Breeding in Plants)
Male-sterile plants provide an important breeding tool for the heterosis of hybrid crops, such as Brassicaceae. In the last decade, circular RNAs (circRNAs), as a novel class of covalently closed and single-stranded endogenous non-coding RNAs (ncRNAs), have received much attention because of their functions as “microRNA (miRNA) sponges” and “competing endogenous RNAs” (ceRNAs). However, the information about circRNAs in the regulation of male-sterility and anther development is limited. In this study, we established the Polima cytoplasm male sterility (CMS) line “Bcpol97-05A”, and the fertile line, “Bcajh97-01B”, in Brassica campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis, and performed RNA expression profiling comparisons between the flower buds of the sterile line and fertile line by whole-transcriptome sequencing. A total of 31 differentially expressed (DE) circRNAs, 47 DE miRNAs, and 4779 DE mRNAs were identified. By using Cytoscape, the miRNA-mediated regulatory network and ceRNA network were constructed, and the circRNA A02:23507399|23531438 was hypothesized to be an important circRNA regulating anther development at the post-transcriptional level. The gene ontology (GO) analysis demonstrated that miRNAs and circRNAs could regulate the orderly secretion and deposition of cellulose, sporopollenin, pectin, and tryphine; the timely degradation of lipids; and the programmed cell death (PCD) of tapetum cells, which play key roles in anther development. Our study revealed a new circRNA–miRNA–mRNA network, which is involved in the anther development of B. campestris, which enriched the understanding of CMS in flowering plants, and laid a foundation for further study on the functions of circRNAs and miRNAs during anther development. View Full-Text
Keywords: cytoplasmic male sterility; CMS; Polima; Brassica campestris; whole-transcriptome sequencing; ceRNA; circRNA; miRNA; anther cytoplasmic male sterility; CMS; Polima; Brassica campestris; whole-transcriptome sequencing; ceRNA; circRNA; miRNA; anther
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MDPI and ACS Style

Liang, Y.; Zhang, Y.; Xu, L.; Zhou, D.; Jin, Z.; Zhou, H.; Lin, S.; Cao, J.; Huang, L. CircRNA Expression Pattern and ceRNA and miRNA–mRNA Networks Involved in Anther Development in the CMS Line of Brassica campestris. Int. J. Mol. Sci. 2019, 20, 4808.

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