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Open AccessArticle

Regulation of Heat Shock Factor Pathways by γ-aminobutyric Acid (GABA) Associated with Thermotolerance of Creeping Bentgrass

Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(19), 4713; https://doi.org/10.3390/ijms20194713
Received: 15 August 2019 / Revised: 13 September 2019 / Accepted: 17 September 2019 / Published: 23 September 2019
(This article belongs to the Section Molecular Plant Sciences)
Activation and enhancement of heat shock factor (HSF) pathways are important adaptive responses to heat stress in plants. The γ-aminobutyric acid (GABA) plays an important role in regulating heat tolerance, but it is unclear whether GABA-induced thermotolerance is associated with activation of HSF pathways in plants. In this study, the changes of endogenous GABA level affecting physiological responses and genes involved in HSF pathways were investigated in creeping bentgrass during heat stress. The increase in endogenous GABA content induced by exogenous application of GABA effectively alleviated heat damage, as reflected by higher leaf relative water content, cell membrane stability, photosynthesis, and lower oxidative damage. Contrarily, the inhibition of GABA accumulation by the application of GABA biosynthesis inhibitor further aggravated heat damage. Transcriptional analyses showed that exogenous GABA could significantly upregulate transcript levels of genes encoding heat shock factor HSFs (HSFA-6a, HSFA-2c, and HSFB-2b), heat shock proteins (HSP17.8, HSP26.7, HSP70, and HSP90.1-b1), and ascorbate peroxidase 3 (APX3), whereas the inhibition of GABA biosynthesis depressed these genes expression under heat stress. Our results indicate GABA regulates thermotolerance associated with activation and enhancement of HSF pathways in creeping bentgrass. View Full-Text
Keywords: antioxidant defense; gene expression; heat shock protein; heat shock factor; oxidative damage; photosynthesis antioxidant defense; gene expression; heat shock protein; heat shock factor; oxidative damage; photosynthesis
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Liu, T.; Liu, Z.; Li, Z.; Peng, Y.; Zhang, X.; Ma, X.; Huang, L.; Liu, W.; Nie, G.; He, L. Regulation of Heat Shock Factor Pathways by γ-aminobutyric Acid (GABA) Associated with Thermotolerance of Creeping Bentgrass. Int. J. Mol. Sci. 2019, 20, 4713.

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