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Open AccessArticle

Induction of Articular Chondrogenesis by Chitosan/Hyaluronic-Acid-Based Biomimetic Matrices Using Human Adipose-Derived Stem Cells

1
Department of Orthopedics, Physical Medicine and Rehabilitation, University Hospital of Munich, 81377 Munich, Germany
2
BioMed Center Innovation gGmbH, 95448 Bayreuth, Germany
3
Department of Heart Surgery, University Hospital of Munich, 81377 Munich, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(18), 4487; https://doi.org/10.3390/ijms20184487
Received: 24 June 2019 / Revised: 6 September 2019 / Accepted: 9 September 2019 / Published: 11 September 2019
Cartilage repair using tissue engineering is the most advanced clinical application in regenerative medicine, yet available solutions remain unsuccessful in reconstructing native cartilage in its proprietary form and function. Previous investigations have suggested that the combination of specific bioactive elements combined with a natural polymer could generate carrier matrices that enhance activities of seeded stem cells and possibly induce the desired matrix formation. The present study sought to clarify this by assessing whether a chitosan-hyaluronic-acid-based biomimetic matrix in conjunction with adipose-derived stem cells could support articular hyaline cartilage formation in relation to a standard chitosan-based construct. By assessing cellular development, matrix formation, and key gene/protein expressions during in vitro cultivation utilizing quantitative gene and immunofluorescent assays, results showed that chitosan with hyaluronic acid provides a suitable environment that supports stem cell differentiation towards cartilage matrix producing chondrocytes. However, on the molecular gene expression level, it has become apparent that, without combinations of morphogens, in the chondrogenic medium, hyaluronic acid with chitosan has a very limited capacity to stimulate and maintain stem cells in an articular chondrogenic state, suggesting that cocktails of various growth factors are one of the key features to regenerate articular cartilage, clinically. View Full-Text
Keywords: chitosan; hyaluronic acid; adipose-derived stem cells; biomaterial; articular chondrogenesis; hTGF-β3; hBMP-6 chitosan; hyaluronic acid; adipose-derived stem cells; biomaterial; articular chondrogenesis; hTGF-β3; hBMP-6
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Huang, Y.; Seitz, D.; König, F.; Müller, P.E.; Jansson, V.; Klar, R.M. Induction of Articular Chondrogenesis by Chitosan/Hyaluronic-Acid-Based Biomimetic Matrices Using Human Adipose-Derived Stem Cells. Int. J. Mol. Sci. 2019, 20, 4487.

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