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Article

Lysates of a Probiotic, Lactobacillus rhamnosus, Can Improve Skin Barrier Function in a Reconstructed Human Epidermis Model

1
College of Pharmacy, Ewha Womans University, Seoul 03760, Korea
2
Severance Biomedical Science Institute, College of Medicine, Yonsei University, Seoul 03722, Korea
3
LCS Biotech, SNU Business Incubator, Suwon 16614, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(17), 4289; https://doi.org/10.3390/ijms20174289
Received: 28 July 2019 / Revised: 22 August 2019 / Accepted: 30 August 2019 / Published: 2 September 2019
(This article belongs to the Special Issue Skin Microbiome)
The main function of the skin is to protect the body from the external environment. The barrier function of the skin is mainly provided by the stratum corneum, which consists of corneocytes bound with the corneodesmosomes and lamellar lipids. Skin barrier proteins like loricrin and filaggrin also contribute to the skin barrier function. In various skin diseases, skin barrier dysfunction is a common symptom, and skin irritants like detergents or surfactants could also perturb skin barrier function. Many efforts have been made to develop strategies to improve skin barrier function. Here, we investigated whether the microfluidized lysates of Lactobacillus rhamnosus (LR), one of the most widely used probiotic species for various health benefits, may improve the skin barrier function in a reconstructed human epidermis, Keraskin™. Application of LR lysate on Keraskin™ increased the expression of tight junction proteins; claudin 1 and occludin as determined by immunofluorescence analysis, and skin barrier proteins; loricrin and filaggrin as determined by immunohistochemistry and immunofluorescence analysis and qPCR. Also, the cytotoxicity of a skin irritant, sodium lauryl sulfate (SLS), was alleviated by the pretreatment of LR lysate. The skin barrier protective effects of LR lysate could be further demonstrated by the attenuation of SLS-enhanced dye-penetration. LR lysate also attenuated the destruction of desmosomes after SLS treatment. Collectively, we demonstrated that LR lysate has protective effects on the skin barrier, which could expand the utility of probiotics to skin-moisturization ingredients. View Full-Text
Keywords: probiotic; Lactobacillus rhamnosus; skin barrier; skin; reconstructed human epidermis model probiotic; Lactobacillus rhamnosus; skin barrier; skin; reconstructed human epidermis model
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MDPI and ACS Style

Jung, Y.-O.; Jeong, H.; Cho, Y.; Lee, E.-O.; Jang, H.-W.; Kim, J.; Nam, K.T.; Lim, K.-M. Lysates of a Probiotic, Lactobacillus rhamnosus, Can Improve Skin Barrier Function in a Reconstructed Human Epidermis Model. Int. J. Mol. Sci. 2019, 20, 4289. https://doi.org/10.3390/ijms20174289

AMA Style

Jung Y-O, Jeong H, Cho Y, Lee E-O, Jang H-W, Kim J, Nam KT, Lim K-M. Lysates of a Probiotic, Lactobacillus rhamnosus, Can Improve Skin Barrier Function in a Reconstructed Human Epidermis Model. International Journal of Molecular Sciences. 2019; 20(17):4289. https://doi.org/10.3390/ijms20174289

Chicago/Turabian Style

Jung, Ye-On, Haengdueng Jeong, Yejin Cho, Eun-Ok Lee, Hye-Won Jang, Jinwook Kim, Ki T. Nam, and Kyung-Min Lim. 2019. "Lysates of a Probiotic, Lactobacillus rhamnosus, Can Improve Skin Barrier Function in a Reconstructed Human Epidermis Model" International Journal of Molecular Sciences 20, no. 17: 4289. https://doi.org/10.3390/ijms20174289

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