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Open AccessArticle

Induction of Necrosis in Human Macrophage Cell Lines by Corynebacterium diphtheriae and Corynebacterium ulcerans Strains Isolated from Fatal Cases of Systemic Infections

1
Department Biologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, Germany
2
Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91054 Erlangen, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to the manuscript (shared first authorship).
Int. J. Mol. Sci. 2019, 20(17), 4109; https://doi.org/10.3390/ijms20174109
Received: 23 July 2019 / Revised: 20 August 2019 / Accepted: 20 August 2019 / Published: 22 August 2019
(This article belongs to the Special Issue Host–Pathogen Interaction)
When infecting a human host, Corynebacterium diphtheriae and Corynebacterium ulcerans are able to impair macrophage maturation and induce cell death. However, the underlying molecular mechanisms are not well understood. As a framework for this project, a combination of fluorescence microscopy, cytotoxicity assays, live cell imaging, and fluorescence-activated cell sorting was applied to understand the pathogenicity of two Corynebacterium strains isolated from fatal cases of systemic infections. The results showed a clear cytotoxic effect of the bacteria. The observed survival of the pathogens in macrophages and, subsequent, necrotic lysis of cells may be mechanisms explaining dissemination of C. diphtheriae and C. ulcerans to distant organs in the body. View Full-Text
Keywords: cell death; FACS; host-pathogen interaction; live cell imaging; macrophages; THP-1 cells cell death; FACS; host-pathogen interaction; live cell imaging; macrophages; THP-1 cells
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Weerasekera, D.; Hahn, J.; Herrmann, M.; Burkovski, A. Induction of Necrosis in Human Macrophage Cell Lines by Corynebacterium diphtheriae and Corynebacterium ulcerans Strains Isolated from Fatal Cases of Systemic Infections. Int. J. Mol. Sci. 2019, 20, 4109.

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