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Open AccessArticle

Glucose Restriction Plus Refeeding in Vitro Induce Changes of the Human Adipocyte Secretome with an Impact on Complement Factors and Cathepsins

Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre, 6200 MD Maastricht, The Netherlands
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Int. J. Mol. Sci. 2019, 20(16), 4055; https://doi.org/10.3390/ijms20164055
Received: 27 June 2019 / Revised: 5 August 2019 / Accepted: 16 August 2019 / Published: 20 August 2019
(This article belongs to the Special Issue Adipokines 2.0)
Adipose tissue is a major endocrine organ capable of secreting adipokines with a role in whole-body metabolism. Changes in the secretome profile during the development of obesity is suspected to contribute to the risk of health complications such as those associated with weight regain after weight loss. However, the number of studies on weight regain is limited and secretome changes during weight regain have hardly been investigated. In an attempt to generate leads for in vivo studies, we have subjected human Simpson Golabi Behmel Syndrome adipocytes to glucose restriction (GR) followed by refeeding (RF) as an in vitro surrogate for weight regain after weight loss. Using LC-MS/MS, we compared the secreted protein profile after GR plus RF with that of normal feeding (NF) to assess the consequences of GR plus RF. We identified 338 secreted proteins of which 49 were described for the first time as being secreted by adipocytes. In addition, comparison between NF and GR plus RF showed 39 differentially secreted proteins. Functional classification revealed GR plus RF-induced changes of enzymes for extracellular matrix modification, complement system factors, cathepsins, and several proteins related to Alzheimer’s disease. These observations can be used as clues to investigate metabolic consequences of weight regain, weight cycling or intermittent fasting. View Full-Text
Keywords: adipokines; SGBS adipocytes; glucose restriction; in vitro fat regain; weight regain; complement factors; cathepsins; extracellular remodeling adipokines; SGBS adipocytes; glucose restriction; in vitro fat regain; weight regain; complement factors; cathepsins; extracellular remodeling
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Qiao, Q.; Bouwman, F.G.; Baak, M.A.; Renes, J.; Mariman, E.C. Glucose Restriction Plus Refeeding in Vitro Induce Changes of the Human Adipocyte Secretome with an Impact on Complement Factors and Cathepsins. Int. J. Mol. Sci. 2019, 20, 4055.

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