Next Article in Journal
Galectin-9 Is a Possible Promoter of Immunopathology in Rheumatoid Arthritis by Activation of Peptidyl Arginine Deiminase 4 (PAD-4) in Granulocytes
Next Article in Special Issue
Transcriptome Profiling Provides Insight into the Genes in Carotenoid Biosynthesis during the Mesocarp and Seed Developmental Stages of Avocado (Persea americana)
Previous Article in Journal
Stem Cells from a Female Rat Model of Type 2 Diabetes/Obesity and Stress Urinary Incontinence Are Damaged by In Vitro Exposure to its Dyslipidemic Serum, Predicting Inadequate Repair Capacity In Vivo
Previous Article in Special Issue
Transgenerational Perpetuation of CHS Gene Expression and DNA Methylation Status Induced by Short Oligodeoxynucleotides in Flax (Linum usitatissimum)
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 20
Issue 16
10.3390/ijms20164045
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Modern Trends in Plant Genome Editing: An Inclusive Review of the CRISPR/Cas9 Toolbox

by
Ali Razzaq
1,
Fozia Saleem
1,
Mehak Kanwal
2,
Ghulam Mustafa
1,
Sumaira Yousaf
2,
Hafiz Muhammad Imran Arshad
2,
Muhammad Khalid Hameed
3,
Muhammad Sarwar Khan
1 and
Faiz Ahmad Joyia
1,*
1
Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad 38040, Pakistan
2
Nuclear Institute for Agriculture and Biology (NIAB), P.O. Box 128, Faisalabad 38000, Pakistan
3
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(16), 4045; https://doi.org/10.3390/ijms20164045
Submission received: 14 June 2019 / Revised: 14 August 2019 / Accepted: 15 August 2019 / Published: 19 August 2019
(This article belongs to the Special Issue Plant Genomics 2019)
Browse Figures
Versions Notes

Abstract

Increasing agricultural productivity via modern breeding strategies is of prime interest to attain global food security. An array of biotic and abiotic stressors affect productivity as well as the quality of crop plants, and it is a primary need to develop crops with improved adaptability, high productivity, and resilience against these biotic/abiotic stressors. Conventional approaches to genetic engineering involve tedious procedures. State-of-the-art OMICS approaches reinforced with next-generation sequencing and the latest developments in genome editing tools have paved the way for targeted mutagenesis, opening new horizons for precise genome engineering. Various genome editing tools such as transcription activator-like effector nucleases (TALENs), zinc-finger nucleases (ZFNs), and meganucleases (MNs) have enabled plant scientists to manipulate desired genes in crop plants. However, these approaches are expensive and laborious involving complex procedures for successful editing. Conversely, CRISPR/Cas9 is an entrancing, easy-to-design, cost-effective, and versatile tool for precise and efficient plant genome editing. In recent years, the CRISPR/Cas9 system has emerged as a powerful tool for targeted mutagenesis, including single base substitution, multiplex gene editing, gene knockouts, and regulation of gene transcription in plants. Thus, CRISPR/Cas9-based genome editing has demonstrated great potential for crop improvement but regulation of genome-edited crops is still in its infancy. Here, we extensively reviewed the availability of CRISPR/Cas9 genome editing tools for plant biotechnologists to target desired genes and its vast applications in crop breeding research.
Keywords: CRISPR/Cas9; genome editing; plant breeding; multiplex genome editing; crop improvement; TALEN; ZFN; biotic stress; abiotic stress CRISPR/Cas9; genome editing; plant breeding; multiplex genome editing; crop improvement; TALEN; ZFN; biotic stress; abiotic stress

Share and Cite

MDPI and ACS Style

Razzaq, A.; Saleem, F.; Kanwal, M.; Mustafa, G.; Yousaf, S.; Imran Arshad, H.M.; Hameed, M.K.; Khan, M.S.; Joyia, F.A. Modern Trends in Plant Genome Editing: An Inclusive Review of the CRISPR/Cas9 Toolbox. Int. J. Mol. Sci. 2019, 20, 4045. https://doi.org/10.3390/ijms20164045

AMA Style

Razzaq A, Saleem F, Kanwal M, Mustafa G, Yousaf S, Imran Arshad HM, Hameed MK, Khan MS, Joyia FA. Modern Trends in Plant Genome Editing: An Inclusive Review of the CRISPR/Cas9 Toolbox. International Journal of Molecular Sciences. 2019; 20(16):4045. https://doi.org/10.3390/ijms20164045

Chicago/Turabian Style

Razzaq, Ali, Fozia Saleem, Mehak Kanwal, Ghulam Mustafa, Sumaira Yousaf, Hafiz Muhammad Imran Arshad, Muhammad Khalid Hameed, Muhammad Sarwar Khan, and Faiz Ahmad Joyia. 2019. "Modern Trends in Plant Genome Editing: An Inclusive Review of the CRISPR/Cas9 Toolbox" International Journal of Molecular Sciences 20, no. 16: 4045. https://doi.org/10.3390/ijms20164045

APA Style

Razzaq, A., Saleem, F., Kanwal, M., Mustafa, G., Yousaf, S., Imran Arshad, H. M., Hameed, M. K., Khan, M. S., & Joyia, F. A. (2019). Modern Trends in Plant Genome Editing: An Inclusive Review of the CRISPR/Cas9 Toolbox. International Journal of Molecular Sciences, 20(16), 4045. https://doi.org/10.3390/ijms20164045

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop