Novel Insights into Regulation of Human Teeth Biomineralization: Deciphering the Role of Post-Translational Modifications in a Tooth Protein Extract
Abstract
:1. Introduction
2. Results and Discussion
2.1. Scanning and Transmission Electron Microscopy (SEM and TEM) Analysis
2.2. Nanoparticle Tracking Analysis (NTA)
3. Materials and Methods
3.1. Isolation of Proteins from Human Teeth
3.2. Dephosphorylation of the Tooth Protein Extract
3.3. Deglycosylation of the Tooth Protein Extract
3.4. Zeta Potential Measurements
3.5. In Vitro Mineralization Assay
3.6. High-Resolution Field Emission Scanning Electron Microscopy (HR FESEM)
3.7. High-Resolution Transmission Electron Microscopy (HRTEM)
3.8. Nanoparticle Tracking Analysis (NTA)
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sharma, V.; Rani, K.; Roychoudhury, A.; Chawla, A.; Nikolajeff, F.; Kumar, S. Novel Insights into Regulation of Human Teeth Biomineralization: Deciphering the Role of Post-Translational Modifications in a Tooth Protein Extract. Int. J. Mol. Sci. 2019, 20, 4035. https://doi.org/10.3390/ijms20164035
Sharma V, Rani K, Roychoudhury A, Chawla A, Nikolajeff F, Kumar S. Novel Insights into Regulation of Human Teeth Biomineralization: Deciphering the Role of Post-Translational Modifications in a Tooth Protein Extract. International Journal of Molecular Sciences. 2019; 20(16):4035. https://doi.org/10.3390/ijms20164035
Chicago/Turabian StyleSharma, Vaibhav, Komal Rani, Ajoy Roychoudhury, Amita Chawla, Fredrik Nikolajeff, and Saroj Kumar. 2019. "Novel Insights into Regulation of Human Teeth Biomineralization: Deciphering the Role of Post-Translational Modifications in a Tooth Protein Extract" International Journal of Molecular Sciences 20, no. 16: 4035. https://doi.org/10.3390/ijms20164035