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Open AccessArticle

Transcriptional Response of Subcutaneous White Adipose Tissue to Acute Cold Exposure in Mice

State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, 100193 Beijing, China
State Key Laboratory of Stem Cell and Reproductive Biology, Chinese Academy of Sciences, 100101 Beijing, China
Authors to whom correspondence should be addressed.
These authors contributed equally to this study.
Int. J. Mol. Sci. 2019, 20(16), 3968;
Received: 28 July 2019 / Revised: 9 August 2019 / Accepted: 13 August 2019 / Published: 15 August 2019
(This article belongs to the Section Molecular Biology)
Beige adipose tissue has been considered to have potential applications in combating obesity and its related metabolic diseases. However, the mechanisms of acute cold-stimulated beige formation still remain largely unknown. Here, transcriptional analysis of acute cold-stimulated (4 °C for 4 h) subcutaneous white adipose tissue (sWAT) was conducted to determine the molecular signatures that might be involved in beige formation. Histological analysis confirmed the appearance of beige adipocytes in acute cold-treated sWAT. The RNA-sequencing data revealed that 714 genes were differentially expressed (p-value < 0.05 and fold change > 2), in which 221 genes were upregulated and 493 genes were downregulated. Gene Ontology (GO) analyses showed that the upregulated genes were enriched in the GO terms related to lipid metabolic process, fatty acid metabolic process, lipid oxidation, fatty acid oxidation, etc. In contrast, downregulated genes were assigned the GO terms of regulation of immune response, regulation of response to stimulus, defense response, etc. The expressions of some browning candidate genes were validated in cold-treated sWAT and 3T3-L1 cell browning differentiation. In summary, our results illustrated the transcriptional response of sWAT to acute cold exposure and identified the genes, including Acad11, Cyp2e1, Plin5, and Pdk2, involved in beige adipocyte formation in mice. View Full-Text
Keywords: beige adipocyte; acute cold stimulation; transcriptional analysis; mice beige adipocyte; acute cold stimulation; transcriptional analysis; mice
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Liang, X.; Pan, J.; Cao, C.; Zhang, L.; Zhao, Y.; Fan, Y.; Li, K.; Tao, C.; Wang, Y. Transcriptional Response of Subcutaneous White Adipose Tissue to Acute Cold Exposure in Mice. Int. J. Mol. Sci. 2019, 20, 3968.

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